Crude oil contamination of soil and water resources is a widespread issue. The present study evaluated the degradation aliphatic hydrocarbons (C 11 –C 36 ) in crude by 17 bacteria isolated from oil–contaminated soil. results suggested that Pseudomonas sp. Bacillus amyloliquefaciens were best hydrocarbon-degrading presence surfactant Tween-80 (0.1% w/v). Based on investigation previous study, + ...

Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivor...

background: much of the environment is affected by petroleum contamination. it imposes serious health problems for humans as well as serious environmental impact. bioremediation is an important consideration for removing environmental pollutants because, compared with other technologies, it incurrs lower costs and is environmentally compatible. methods: crude oil degrading bacteria were isolate...

The influence of oil concentration on hydrocarbon biodegradation in a sandy sediment was studied in polyvinyl chloride reactors (0.45 x 0.28 x 0.31 m) containing 76.8 kg of beach sand in natura, where the upper layer was artificially contaminated with petroleum. The oil-degrading microorganisms used consisted of a mixed culture named ND, obtained from landfarming and associated with indigenous ...

The gastrointestinal (GI) habitat of ruminant and non-ruminant animals sustains a vast ensemble of microbes that are capable of utilizing lignocellulosic plant biomass. In this study, an indigenous swine (Zovawk) and a domesticated goat (Black Bengal) were investigated to isolate bacteria having plant biomass degrading enzymes. After screening and enzymatic quantification of eighty-one obtained...

Nutrient addition has been proved to be an effective strategy to enhance oil biodegradation in marine shorelines. To determine the optimal range of nutrient concentrations in the bioremediation of oil-polluted beaches, nitrate was added to the simulated shoreline models in the initial concentration of 1, 5 and 10 mg/L. Whenever the NO3-N concentration declined to 70% of its original value, addi...

One of the major environmental concerns of the Deepwater Horizon oil spill in the Gulf of Mexico was the ecological impact of the oil that reached shorelines of the Gulf Coast. Here we investigated the impact of the oil on the microbial composition in beach samples collected in June 2010 along a heavily impacted shoreline near Grand Isle, Louisiana. Successional changes in the microbial communi...

Microbial Enhanced Oil Recovery (MEOR) is a tertiary oil recovery method that utilizes microbes to enhance oil production. This research was focused on the isolation and characterization of indigenous bacteria from a South Sumatra’s oilfield which were able to degrade heavy crude oil and decrease oil viscosity. The total of 33 colonies were successfully isolated based on sequential isolation me...

poly aromatic hydrocarbons are hydrophobic organic compound found as major contaminants in soil. soil biological activities are vital for the restoration of soil contaminated with hydrocarbons. successful application of bioremediation technology to contaminated soil requires knowledge of the characteristics of the site and the parameters that affect the microbial biodegradation of pollutants. t...