Background: Ferulago angulata is an important medicinal plant and endemic to Iran which is an endangered Plant. Objective: The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat. Metthods: Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was ...

Background: Polycystic ovary syndrome (PCOS) is a multifactorial, heterogeneous, complex genetic endocrine and metabolic disorder, diagnostically characterized by chronic anovulation, polycystic ovaries and biochemical and clinical manifestations of hyperandrogenism. In patients with PCOS lower apoptotic rates found in granulosa cells compared to women with regular ovulatory cycles and this lea...

Improvements in in vitro maturation techniques have focused on culture optimization to increase oocyte maturation rates. Specialized culture media, now commercially available, did not perform significantly better than standard IVF culture media for maturation of immature oocytes in our normal IVF cases.

background & objectives : different energy substrates and nutrients can greatly influence oocyte nuclear and cytoplasmic maturation. effect of glucose on in-vitro oocyte maturation and subsequent early embryonic development is controversial. the objective of the present study was to verify the effect of various concentrations of glucose on cumulus-oocyte complexes maturation, fertilization and ...

Background: In vitro maturation and fertilization are techniques to increase efficiency of mammals reproduction. However, in vitro culture conditions exert an imbalance between oxidants and antioxidants which may reduce live birth and pregnancy rates when compare to in vivo conditions. Materials and Methods: The present study was conducted as a part of the ovine IVF program organized by Univers...

background: the effect of temperature and humidity on the maturation of toxocara cati eggs in an in vitro system was investigated. methods: suspensions of toxocara cati eggs, with 5% formalin/saline or 2.5% formalin/ringer were prepared and maintained at 37 °c under 40% humidity or at 25 °c under 98% humidity for 3 weeks for egg development. results: the suspension sample mixed by 2.5% formalin...

Background: The use of C-type natriuretic peptide (CNP) combined with cysteamine during pre-in vitro maturation (IVM) can help establish an effective pre-IVM system. Aims: This study was designed to investigate the effects of CNP combined with cysteamine on meiotic maturation and developmental competence of bovine oocytes in vitro. Met...

this study was inducted to elucidate the effect of different steroid hormones on simultaneous maturation of oocytes in caspian salmon. oocytes were incubated with 1, 10, 100 and 1000 ng/ml of testosterone, progesterone and 17 β stradiol, in vitro, for 40 hrs. gvbd was considered as hormones effectiveness. 17β estradiol was effective just in 1000 ng/ml and testosterone had no positive effect on ...

Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups....