Determining starch degradability is important because of its implicit relation to gut health, ruminal acidosis, energy availability, feed intake, and animal productivity. In vitro assays for protein fiber have been useful tools ration formulation; however, analogous assessments limited update. Our objective was test how an enzymatic in degradation assay compared with the situ technique terms ab...

The exosome complex is involved in multiple RNA processing and degradation pathways. How exosome is recruited to particular RNA substrates and then chooses between RNA processing and degradation modes remains unclear. We find that the RNA binding protein Nrd1, complexed with its partners Nab3, Sen1, and cap binding complex, physically interacts with the nuclear form of exosome. Nrd1 stimulates ...

RNA decay is usually mediated by protein complexes and can occur in specific foci such as P-bodies in the cytoplasm of eukaryotes. In human mitochondria nothing is known about the spatial organization of the RNA decay machinery, and the ribonuclease responsible for RNA degradation has not been identified. We demonstrate that silencing of human polynucleotide phosphorylase (PNPase) causes accumu...