Graphene oxide/Fe₃O₄ (GO/Fe₃O₄) nanoparticles were synthesized by an ultrasonic-assisted reverse co-precipitation method, and then horseradish peroxidase (HRP) was covalently immobilized onto GO/Fe₃O₄ with 1-ethyl-3-(3-dimethyaminopropyl)carbodiimide (EDC) as a cross-linking agent. In order to enhance the phenol removal efficiency and prevent the inactivation of the enzyme, the polyethylene gly...

Alcohol dehydrogenase from Bacillus (Geobacillus) stearothermophilus (BsADH) is a NADH-dependent enzyme catalyzing the oxidation of alcohols, however its thermal and operational stabilities are too low for long-term use under non-physiological conditions. Enzyme immobilizations emerges as an attractive tool to enhance stability this enzyme. In work, we have screened battery porous carriers immo...

Penicillin acylase, an industrially important biocatalyst catalyzes the conversion of penicillins to 6-amino penicillanic acid (6-APA) which is the main precursor for the production of semisynthetic ß-lactam antibiotics. The present work involves the continuous production of 6APA in a packed column reactor by using agarose immobilized penicillin acylase as a block polymer. The strain Escherichi...

A novel and facile method for the preparation of an enzyme-immobilized microreactor has been developed in which enzymes are immobilized as an enzyme-polymer membrane formed on the inner wall of the microchannel by a cross-linking polymerization method; the resulting microreactor shows excellent reaction performance and stability against denaturating agents.

The development of coimmobilized multi-enzymatic systems is increasingly driven by economic and environmental constraints that provide an impetus to develop alternatives to conventional multistep synthetic methods. As in nature, enzyme-based systems work cooperatively to direct the formation of desired products within the defined compartmentalization of a cell. In an attempt to mimic biology, c...

An enzyme electrode was developed using the enzyme sulfite oxidase (EC1.8.3.1) immobilized onto pig intestine. Hydrogen peroxide produced was monitored amperometrically. All experimental parameters such as pH, temperature, buffer constituent concentration, were thoroughly investigated and optimized when appropriate. The response to sulfite concentration was linear in the range 5.2´...

A novel enzyme-immobilized flow-through interface was designed for sensitive end-column chemiluminescent (CL) detection in open-tubular capillary electrochromatography (OTCEC). Enzyme was covalently bound on an aldehyde-activated polymer membrane immobilized in a flow cell to catalyze the CL reaction that occurred in it. Using glycine as the model analyte, N-(4-aminobutyl)-N-ethylisoluminol-der...

Keratinases are a group of proteolytic enzymes that display the capability of degrading insoluble keratin substrates such as feather resulting as poultry waste. Entrapment of crude/purified enzyme within a matrix is a common method for immobilization. An immobilized enzyme provides increased resistance to changes in conditions such as pH or temperature. It also allows enzymes to be held in plac...

The purified and thermostable -amylase from soybean seeds was immobilized by entrapment on agarose and agar matrices and their catalytic properties were compared. The optimum pH of -amylase immobilized on both matrices was 7.0. The 1% of agarose (w/v) and 4% of agar (w/v) yielded an optimum immobilization of about 75 and 77% respectively. The K and V values as determined from the GraphPad m max...

Cellulase was immobilized onto silica gel surfaces pretreated with (3-aminopropyl) triethoxy-silane (3-APTES), and glutaraldehyde (GA) was used as a cross-linker. A carboxymethyl cellulose sodium salt (CMC) solution was used for activity experiments. Protein assay was performed to determine the mass immobilized and compare with free enzyme. Cellulase was successfully demonstrated to be immobili...