Using Shigella flexneri 2a YSH6000, we isolated 304 independent Tn5 insertion mutants in the 230-kilobase invasion plasmid, pMYSH6000. The site of each Tn5 insertion was assigned to 23 SalI fragments on the previously made SalI cleavage map of pMYSH6000. Among the 304 insertions, 150 were negative in expression of four phenotypes examined (mouse Sereny test [Ser], invasion into epithelial cells...

We described previously a CTL clone able to lyse the autologous carcinoma cell line IGR-Heu after specific recognition of an HLA-A2/mutated alpha-actinin-4 peptide complex. Here, we used IGR-Heu, cultured either as standard two-dimensional monolayers or as three-dimensional spheroids, to further analyze the influence of target architecture on CTL reactivity. Interestingly, we found that changes...

Aims. We aim to establish the X-ray properties of the intermediate polar candidate IGR J15094–6649 and therefore confirm its inclusion into the class. Methods. 42 856 s of X-ray data from RXTE was analysed. Frequency analysis was used to constrain temporal variations and spectral analysis used to characterise the emission and absorption properties. Results. A spin period of 809.7 ± 0.6 s is pre...

By random transposon Tn5 insertions, we previously identified six virulence-associated SalI fragments, B, D, F, G, H, and P, in the 230-kilobase plasmid pMYSH6000 of Shigella flexneri 2a. In this study, we analyzed the sites of 134 independent Tn5 insertions on four contiguous SalI fragments, B, P, H, and D, of pMYSH6000 and identified five virulence-associated regions; four were associated wit...

Comparisons of the relative activities of 11 intergenic region (IGR) internal ribosome entry site (IRES) elements of insect dicistrovirus with 5' IRES elements of the hepatitis C and encephalomyocarditis viruses were performed in insect and mammalian cells. Dual luciferase assays were performed to determine the most effective dicistrovirus IGR IRES in the lepidopteran cell lines Sf9 (Spodoptera...

OBJECTIVE To report the age- and sex-specific prevalence of diabetes and impaired glucose regulation (IGR) according to revised World Health Organization criteria for diabetes in Asian populations. RESEARCH DESIGN AND METHODS We performed 11 studies of 4 countries, comprising 24,335 subjects (10,851 men and 13,484 women) aged 30-89 years who attended the 2-h oral glucose tolerance test and me...

We report on optical and X–ray observations of the accretion powered ms pulsar IGR J00291+5934 in quiescence. Time resolved I–band photometry has been obtained with the 4.2 m William Herschel Telescope, while a 3 ks Chandra observation provided contemporaneous X–ray coverage. We found an unabsorbed 0.5–10 keV X–ray flux of 1×10 erg cm s which implies that the source was in quiescence at the tim...

We described previously a CTL clone able to lyse the autologous carcinoma cell line IGR-Heu after specific recognition of an HLA-A2/ mutated -actinin-4 peptide complex. Here, we used IGR-Heu, cultured either as standard two-dimensional monolayers or as three-dimensional spheroids, to further analyze the influence of target architecture on CTL reactivity. Interestingly, we found that changes in ...

We report on the identification of a new soft gamma-ray source, IGR J12319–0749, detected with the IBIS imager on board the INTEGRAL satellite. The source, which has an observed 20−100 keV flux of ∼8.3 × 10−12 erg cm−2 s−1, is spatially coincident with an active galactic nucleus (AGN) at redshift z = 3.12. The broad-band continuum, obtained by combining XRT and IBIS data, is flat (Γ = 1.3) with...

An attempt was made to assess the control potency of an insect growth regulator (IGR), hexaflumuron, against Aedes albopictus, a potent dengue vector, both in the laboratory and under field conditions. Emergence inhibition activity of this IGR against laboratory reared and field collected larvae of Ae. albopictus showed EI50 values of 1.9 x 10(-4) and 1.80 x 10(-4) mg(ai)/l respectively. Under ...