The Image Foresting Transform (IFT) is a framework for seeded image segmentation, based on the computation of minimal cost paths in a discrete representation of an image. In two recent publications, we have shown that the segmentations obtained by the IFT may be improved by refining the segmentation locally around the boundaries between segmented regions. Since these methods operate on a small ...

We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-alpha-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriol...

Intraflagellar Transport (IFT) refers to a highly conserved process occurring in eukaryotic ciliated structures. In vertebrate photoreceptors, IFT mediates protein trafficking to the outer segments. The IFT particle is a multi-subunit complex and mutations in many individual components causes photoreceptor defects. In zebrafish, mutations in the ift57, ift88, and ift172 genes result in retinal ...

PURPOSE To identify the mutation and cell biological underpinnings of photoreceptor defects in zebrafish au5 mutants. METHODS Whole genome sequencing and SNP mapping were used to determine the genomic interval that harbors the au5 mutation. A candidate mutation was cloned and sequenced, and mRNA rescue used to validate that the affected gene was correctly identified. In situ hybridization, im...

Purpose IFT81, a core component of the IFT-B complex, involved in the bidirectional transport of ciliary proteins, has been recently implicated in syndromic ciliopathies. However, none of the IFT-B core complex proteins have been associated with nonsyndromic retinal dystrophies. Given the importance of ciliary transport in photoreceptor function and structural maintenance, we sought to investig...

Intraflagellar transport (IFT), the key mechanism for ciliogenesis, involves large protein particles moving bi-directionally along the entire ciliary length. IFT particles contain two large protein complexes, A and B, which are constructed with proteins in a core and several peripheral proteins. Prior studies have shown that in Chlamydomonas reinhardtii, IFT46, IFT52, and IFT88 directly interac...

Primary cilia are specialised sensory and developmental signalling devices extending from the surface of most eukaryotic cells. Defects in these organelles cause inherited human disorders (ciliopathies) such as retinitis pigmentosa and Bardet-Biedl syndrome (BBS), frequently affecting many physiological and developmental processes across multiple organs. Cilium formation, maintenance and functi...

The interfacial tension (IFT) between aluminium and cryolite melts containing different salt additions (AlF3, NaF, Na2SO4) has been measured during electrolysis by the capillary depression method. The technique is based on the measurement of the capillary depression occurring when a capillary, which is moved vertically down through the molten salt layer, passes through the metal/salt interface....

This paper presents DE/IFT, a new fault diagnosis engine which is based on the authors’ IFT algorithm for induction of fault trees. It learns from an examples database comprising sensor recordings, all of which have been classified as corresponding to either the normal behaviour of the system or to one or more fault states. The fault trees generated by IFT are translated into functions in the C...