An efficient Agrobacterium-mediated genetic transformation protocol has been developed in the present investigation to integrate salinity tolerant gene pea DNA helicase (PDH45) two peanut varieties, Dhaka-1 and BARI Chinabadam-8. Decapitated half-embryo explants of above-mentioned varieties were also used develop a compatible vitro regeneration system. MS medium supplemented with 5.0 mg/l BAP 0...

Agrobacterium-mediated genetic transformation was explored for inducing insect resistance in mat rush (Juncus effusus L.). Firstly, the callus regeneration system was established, and secondly the Agrobacterium tumefaciens strain EHA105 with plasmid pKUB carrying cryIA(b), hph and gus genes, coding for hygromycin phosphotransferase and β-glucuronidase, respectively, was used for transformation....

Most plastid transformation studies with tobacco, and all reports for other species (except tomato [G.D. Nugent, M. ten Have, A. van der Gulik, P.J. Dix, B.A. Uijtewaal, A.P. Mordhorst, Plastid transformants of tomato selected using mutations affecting ribosome structure. Plant Cell Rep. 24 (2005) 341–349]), have used biolistics for plastid transformation. However, nuclear transformation via bi...

Hepatitis delta virus (HDV) infection and spread in vivo are dependent upon coinfection by hepatitis B virus (HBV), and dual HDV/HBV infection is frequently more severe than HBV infection alone, raising the possibility that HDV infection may be deleterious to cells. Here we have examined the effects of HDV replication on the long-term growth of cultured cells. Our results show that most cells t...

Collards (Brassica oleracea var. acephala) are an important vegetable crucifer produced worldwide for human consumption, and one subject to severe injury by Lepidoptera. We have produced Bacillus thuringiensis (Bt)-transgenic collard lines that have the potential to be used either for direct control or as a ‘‘dead end’’ trap crop for Lepidoptera. To produce collard lines expressing Bt genes, a ...

abstract lettuce (lactuca sativa l.) is a major leafy vegetable and belongs to the asteraceae family (compositae). the genetic manipulation of lettuce requires a reliable and efficient regeneration method. since lettuce is a suitable bioreactor for production of recombinant proteins especially edible vaccines. this study was conducted to identify an appropriate method for lettuce gene transform...