BACKGROUND Vitamin D insufficiency has been implicated in autoimmunity. ChIP-seq experiments using immune cell lines have shown that vitamin D receptor (VDR) binding sites are enriched near regions of the genome associated with autoimmune diseases. We aimed to investigate VDR binding in primary CD4+ cells from healthy volunteers. METHODS We extracted CD4+ cells from nine healthy volunteers. E...

This multicenter multinational study, in children with high genetic risk of T1D, identified an association between lower serum 25-hydroxyvitamin D (25OHD) concentrations and subsequent appearance islet autoimmunity or T1D.

BACKGROUND Improvements in antenatal vitamin D status may have maternal-infant health benefits. To inform the design of prenatal vitamin D3 trials, we conducted a pharmacokinetic study of single-dose vitamin D3 supplementation in women of reproductive age. METHODS A single oral vitamin D3 dose (70,000 IU) was administered to 34 non-pregnant and 27 pregnant women (27 to 30 weeks gestation) enr...

Despite major advances in intensive care, sepsis continues to be a major cause of morbidity and mortality. Vitamin D is involved in various physiologic functions, including cellular responses during infection and inflammation. The aim of this study was to evaluate diagnostic value of 25-hydroxyvitamin D in childhood sepsis because it can be fatal if diagnosis delayed. The study included 40 chil...

OBJECTIVES: Recent studies have shown a high prevalence of hypovitaminosis D, defined as a serum 25-hydroxyvitamin D level less than 30 ng/ml, in both healthy populations and patients with chronic kidney disease. Patients undergoing kidney transplant are at an increased risk of skin cancer and are advised to avoid sunlight exposure. Therefore, these patients might share two major risk factors f...

we investigated the association between serum 25(oh) d levels and depressive symptoms in patients with major depressive disorder (mdd).eighty-five adults, 44 drug free patients with mdd and 41 apparently healthy controls, participated in the study. the hamilton depression rating scale was used to assess severity of major depression. mental health of the controls was assessed according to dsm-iv...

Am J Clin Pathol 2012;137:831-835 833 © American Society for Clinical Pathology The Author’s Reply I read with interest the comments by Arya and Agarwal who broadened the discussion of vitamin D testing into an international context and raised a number of important points. First, 25-hydroxyvitamin D testing is currently not accessible in many parts of the world owing to financial constraints or...

BACKGROUND Inadequate vitamin D status is common in many populations around the world. OBJECTIVE The aim was to evaluate potentially modifiable determinants of vitamin D status in an older population. DESIGN This was a cross-sectional study from a population-based cohort including 538 white Dutch men and women aged 60-87 y. Vitamin D status was assessed by plasma 25-hydroxyvitamin D [25(OH)...

Introduction: In serum, 25-hydroxy-vitamin D (25(OH)D) can be found in two forms, namely 25(OH)D2 and 25(OH)D3. We recently published a mathematical method to estimate the 25(OH)D2 recovery without spiking the samples. Since then, new “total” vitamin D immunoassays have appeared on the market (Roche “Total” vitamin D, Siemens Centaur vitamin D “total”, “total” vitamin D on DiaSorin Liaison XL, ...

[This corrects the article DOI: 10.1155/2014/691679.].