Addition of the four deoxyribonucleosides in vitro to 6C3HED mouse ascites tumor stimulates 32P incorporation into DNA and depresses incorporation into RNA at concentrations greater than 10~4 M. The four ribonucleosides stimulate incorporation into both DNA and RNA. Hydroxyurea blocks the ribonucleoside stimulation of 32P incorporation into DNA but not RNA. The inhibition of 32P incorporation i...

Addition of the four deoxyribonucleosides in vitro to 6C3HED mouse ascites tumor stimulates 32P incorporation into DNA and depresses incorporation into RNA at concentrations greater than 10~4 M. The four ribonucleosides stimulate incorporation into both DNA and RNA. Hydroxyurea blocks the ribonucleoside stimulation of 32P incorporation into DNA but not RNA. The inhibition of 32P incorporation i...

Inhibition of DNA replication with hydroxyurea during thymine starvation of Escherichia coli shows that active DNA synthesis is not required for thymineless death (TLD). Hydroxyurea experiments and thymine starvation of lexA3 and uvrA DNA repair mutants rule out unbalanced growth, the SOS response, and nucleotide excision repair as explanations for TLD.

In order to seek a urease inhibitor more potent than hydroxyurea (1), its alkyl- or phenyl-substituted derivatives were synthesized and evaluated for their effect on the jack bean urease. Of 16 compounds tested, m-methyl- (10) and m-methoxy-phenyl substituted hydroxyurea (13) showed the most potent inhibitory activities against the enzyme.

The following comix>unds inhibited incorporation of thymidine-3H into the DNA of HeLa cells without significantly impairing cellular incorjwration of uridine-3H into RNA or loucine-3H into protein; the}' are listed in order of potency. Dihydro.xyurea > jV-methylhydroxyurea > .V-acetylhydroxyurea = hydroxyurea = A'-hydroxyguanidine = N-hydroxyurethane = AT-ethylhydroxyurea > 3-phenyl-l-hydroxyur...

In budding yeast Saccharomyces cerevisiae, kinetochores are attached by microtubules during most of the cell cycle, but the duplication of centromeric DNA disassembles kinetochores, which results in a brief dissociation of chromosomes from microtubules. Kinetochore assembly is delayed in the presence of hydroxyurea, a DNA synthesis inhibitor, presumably due to the longer time required for centr...

Hydroxyurea and higher hemoglobin F improve the clinical course and survival in sickle cell disease, but their roles in protecting from pulmonary hypertension are not clear. We studied 399 children and adolescents with sickle cell disease at steady state; 38% were being treated with hydroxyurea. Patients on hydroxyurea had higher hemoglobin concentration and lower values for a hemolytic compone...

BACKGROUND We investigated adhesion receptor levels on red blood cells, reticulocytes and erythroid progenitors from children with sickle cell disease treated or not with hydroxyurea. DESIGN AND METHODS Four groups of patients were investigated: (i) children receiving hydroxyurea for severe vaso-occlusive events (n=26); (ii) untreated children with a history of vaso-occlusive events (n=20); (...

Our previous study showed a reduction in serum ferritin of β-thalassemia patients on hydroxyurea therapy. Here we aimed to evaluate the efficacy of hydroxyurea alone and in combination with most widely used iron chelators like deferiprone and deferasirox for reducing iron from experimentally iron overloaded mice. 70 BALB/c mice received intraperitonial injections of iron-sucrose. The mice were ...

Many anticancer agents damage DNA and arrest cell-cycle progression primarily in S or G2 phase of the cell cycle. Previous studies with the topoisomerase I inhibitor SN38 have shown the efficacy of the Chk1 inhibitor UCN-01 to overcome this arrest and induce mitotic catastrophe. UCN-01 was limited in clinical trials by unfavorable pharmacokinetics. SCH900776 is a novel and more selective Chk1 i...