The activities of three enzymes concerned with collagen metabolism 4-prolyl hydroxylase, UDP-glucose: collagen glucosyltransferase and glucosyl-galactosyl-hydroxylysine glucohydrolase and 4-hydroxyproline content have been studied in the cardiac ventricles of spontaneously hypertensive rats (SHR) during prehypertensive, hypertensive and sustained hypertensive stages (respectively 4.5, 12 and 19...

This study investigates the feasibility of recombinant spider silk protein as a wound-dressing material for coverage of deep second-degree burn wounds using an animal model. Sixty Sprague-Dawley (SD) rats were randomly divided into four groups (15 rats in each group). Two types of recombinant spider silk proteins, pNSR-16 and pNSR-32, as well as collagen (as a control) were applied on the wound...

Modern age exposes humans to an increasing level of electromagnetic activity in their environment due to overhead power lines and transformers around residential areas. Studies have shown that treatment with antioxidants can suppress the oxidative damage induced by electromagnetic fields in various frequencies of the non-ionizing radiation band. In this study, we detected protein carbonyl conte...

OBJECTIVE To determine the biochemical changes in articular cartilage composition associated with the development of avian degenerative joint disease (DJD) in ad libitum fed broiler fowl, in comparison to feed-restricted broilers and J-lin fowl (non-susceptible to DJD). METHODS Articular cartilage from the distal tibiotarsus (DTT) was characterised up to age 180 days. Proteoglycan content was...

The hydroxyproline/creatinine ratio for two groups of healthy individuals and two groups of patients with a history of mammary and prostatic carcinoma without metastases was measured and compared with literature values. The hydroxyproline/creatinine ratio for healthy adults appears to be 1.4 (range 0.7--2.3). Furthermore, the influence of diet and gelatin load of 30 g on the hydroxyproline crea...

The main protein in alveolar bone and dentin is collagen.' Two thirds of the total amino acids found in collagen are composed of glycine, alanine, proline, and hydroxyproline.' The hydroxyproline in collagen is derived almost exclusively from hydroxylation of proline. Thus the proline molecule may enter collagen in two ways: as proline or as hydroxyproline.2 4 Many studies have used radioactive...

The urinary excretion of hydroxyproline, measured as the hydroxyproline: creatinine ratio, was useful in monitoring the progression of metastatic cancer of the breast. After new treatment was started changes in the hydroxyproline excretion occurred earlier than other clinically observable responses. The test could therefore be used for predicting the response to treatment and early detection of...

A study has been made of the effects on hydroxyproline formation of 4 factors that influence the rate of cell elongation in the Avena coleoptile; auxin, sugars, an external osmoticum, and actinomycin D. Hydroxyproline formation is increased by a combination of auxin and sucrose, but is affected to a much lesser extent by either factor alone. Its formation is inhibited by an external osmoticum b...