Cytosolic and mitochondrial aspartate aminotransferase cDNAs were cloned from a lambda gt11 rat liver cDNA library. The complete coding sequence and the 3' non-coding sequence of the cytosolic isozyme mRNA were obtained from two overlapping cDNA clones. Partial sequences of the mitochondrial enzyme cDNAs were found to be identical to the recently published complete sequence (Mattingly, J. R., J...

Inhibition of liver aspartate aminotransferase by L-2-amino-4-methoxy-trans-3-butenoic acid in the suckling newborn rat causes a decrease in all gluconeogenic precursors from phosphoenolpyruvate to glucose and an accumulation of lactate but not of pyruvate. This suggests that the aspartate shuttle is operative and confirms the quantitative importance of lactate as a gluconeogenic precursor at t...

In the present study, we examined the antioxidative activities of Satureja khuzestanica essential oil (SKE) and possible protective effect of SKE on lipid profile, atherogenic index and liver enzyme markers in Alloxan-induced Type 1 diabetic rats. Thirty male rats were randomly divided into three groups; group one as control, group two diabetic untreatment, and group three treatments with SKE b...

The catalytic activity concentrations of aspartate aminotransferase (EC 2.6.1.1) and alanine aminotransferase (EC 2.6.1.2) in several commercial control sera were determined at three different temperatures. Although these sera were of various biological origins, the temperature-conversion factors calculated are nearly identical to those for native human sera. The proportional increase of the ac...

This study was designed to investigate the hepatotoxicity of ranitidine treatment in dose levels of 10, 30, and 50 mg/kg b.wt. for 3 weeks period in male rats. The results showed some adverse changes in rats treated with either 10 or 30 mg/kg. Treatment with dose of 50 mg/kg produced marked increase in the activity of both acid phosphatase in liver and aspartate aminotransferase in serum and li...

INTRODUCTION To raise awareness among health care workers of the risk of withdrawal symptoms after longstanding and intense abuse of gamma-hydroxybutyric acid. CASE PRESENTATION A 23 year old Caucasian woman presented with gamma-hydroxybutyric addiction and withdrawal syndrome. The symptoms of gamma-hydroxybutyric withdrawal in this patient initially went unrecognized, upon which her situatio...

Organelles in spinach leaves were isolated in sucrose gradients by isopycnic centrifugation. The peroxisomal fraction was the only cellular site for two different and irreversible aminotransferases which utilized glyoxylate as the amino acceptor in the formation of glycine. These were a serine:glyoxylate aminotransferase with a specific activity of 1.54 pmoles X min-1 X mg-1 peroxisomal protein...

the present study investigated the possible protective effect of helicteres isora (sterculiaceae) bark extracts on certain biochemical markers in streptozotocin (stz)-induced diabetes in rats. stz treatment (60 mg/kg/i.p) caused a hyperglycemic state that led to various physiological and biochemical alterations. blood levels of glucose, urea, uric acid and creatinine, plasma levels of albumin a...

the efficacy and safety of salvia officinalis combined with statin have not been evaluated in dyslipidemic diabetes mellitus type 2 (ddmt2) so far. the plant extract antioxidant activity was determined by the dpph radical scavenging assay. the total flavonoid, total phenolic and quercetin contents of the capsules containing the plant extract were also measured. moreover, the effects of 2-month ...