نتایج جستجو برای: hydroxybutyrate phb

تعداد نتایج: 4992  

2009
D Rohini S Phadnis S K Rawal

A poly-β-hydroxybutyrate (PHB) accumulating, Gram positive Bacillus thuringiensis R1 was isolated from the soil samples. The growth of the organism and PHB accumulation in the presence of different carbon sources was studied. While maximum dry cell mass accumulation (3.90 to 4.11g/L) resulted in the presence of glycerol (1% v/v), least biomass formation (1.28g/L) was in the presence of 1%(v/v) ...

2016
Luis P. S. Alves Cícero S. Teixeira Evandro F. Tirapelle Lucélia Donatti Michelle Z. Tadra-Sfeir Maria B. R. Steffens Emanuel M. de Souza Fabio de Oliveira Pedrosa Leda S. Chubatsu Marcelo Müller-Santos

Phasins are important proteins controlling poly-3-hydroxybutyrate (PHB) granules formation, their number into the cell and stability. The genome sequencing of the endophytic and diazotrophic bacterium Herbaspirillum seropedicae SmR1 revealed two homologous phasin genes. To verify the role of the phasins on PHB accumulation in the parental strain H. seropedicae SmR1, isogenic strains defective i...

Journal: :British journal of plastic surgery 2002
R C Young M Wiberg G Terenghi

The repair of peripheral nerve injuries with an autologous nerve graft is still the treatment of choice, although it is widely recognised that the method is associated with donor-site morbidity and a suboptimal functional outcome. An alternative approach is to use a nerve conduit to provide a guidance channel for the regenerating nerve. This study investigates the potential of poly-3-hydroxybut...

Journal: :Applied and environmental microbiology 2007
Jing Han Qiuhe Lu Ligang Zhou Jian Zhou Hua Xiang

Although many haloarchaea produce biodegradable polyhydroxyalkanoates (PHAs), the genes involved in PHA synthesis in the domain of Archaea have not yet been experimentally investigated yet. In this study, we revealed that Haloarcula marismortui was able to accumulate poly(3-hydroxybutyrate) (PHB) up to 21% of cellular dry weight when cultured in a minimal medium with excessive glucose and ident...

Journal: :Applied and environmental microbiology 2006
Pablo I Nikel M Julia Pettinari Miguel A Galvagno Beatriz S Méndez

We assessed the effects of different arcA mutations on poly(3-hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some of the characteristics of the Arc phenotype which confers high respiratory capacity. PHB synthesis...

Journal: :International microbiology : the official journal of the Spanish Society for Microbiology 2005
Pablo I Nikel M Julia Pettinari Beatriz S Méndez Miguel A Galvagno

A statistically based Plackett-Burman screening design identified milk whey and corn steep liquor concentrations as well as ionic strength (based on phosphate buffer concentration) as the three main independent components of the culture medium that significantly (p < 0.05) influenced biomass and poly(3-hydroxybutyrate) (PHB) production in recombinant cells of Escherichia coli. This strain carri...

Journal: :Journal of bacteriology 1991
P Schubert N Krüger A Steinbüchel

Molecular methods have been applied to analyze the expression of the Alcaligenes eutrophus poly(3-hydroxybutyrate) (PHB) synthase gene (phbC). The translational initiation codon was identified by analysis of the amino acid sequence of a PHB synthase-beta-galactosidase fusion protein. This protein was purified to almost gel electrophoretic homogeneity by chromatography on DEAE-Sephacel and on am...

2012
Alessandra Zonari Silviene Novikoff Naira R. P. Electo Natália M. Breyner Dawidson A. Gomes Albino Martins Nuno M. Neves Rui L. Reis Alfredo M. Goes

Tissue engineering is based on the association of cultured cells with structural matrices and the incorporation of signaling molecules for inducing tissue regeneration. Despite its enormous potential, tissue engineering faces a major challenge concerning the maintenance of cell viability after the implantation of the constructs. The lack of a functional vasculature within the implant compromise...

Journal: :Applied and environmental microbiology 2009
Mohammad H A Ibrahim Alexander Steinbüchel

Industrial production of biodegradable polyesters such as polyhydroxyalkanoates is hampered by high production costs, among which the costs for substrates and for downstream processing represent the main obstacles. Inexpensive fermentable raw materials such as crude glycerol, an abundant by-product of the biodiesel industry, have emerged to be promising carbon sources for industrial fermentatio...

Journal: :Journal of bacteriology 2002
Natalia Korotkova Ludmila Chistoserdova Mary E Lidstrom

Methylobacterium extorquens AM1, a serine cycle facultative methylotroph, accumulates poly-beta-hydroxybutyrate (PHB) as a carbon and energy reserve material during growth on both multicarbon- and single-carbon substrates. Recently, the identification and mutation of the genes involved in the biosynthesis and degradation of PHB have been described for this bacterium, demonstrating that two of t...

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