Although it has been conclusively demonstrated in numerous reports that acetate and mevalonic acid are converted to squalene or sterols (3-lo), it has not been proved that mevalonic acid is formed from acetate by a mammalian enzyme system. Evidence has been presented, however, for the formation of this compound from P-hydroxy, P-methyl glutaryl coenzyme A in the presence of reduced triphosphopy...

Long-chain alkanehydroxamic acids adsorb on the native oxides of metals and formed oriented selfassembled monolayers (SAMs). This study examined SAMs of hydroxamic acids on the native oxides of copper, silver, titanium, aluminum, zirconium, and iron. These SAMs were characterized usingwettability, X-ray photoelectron spectroscopy (XPS), and polarized infrared external reflectance spectroscopy (...

In the present study, the ability of the hydroxamate histone deacetylase (HDAC) inhibitor 4-Me2N-BAVAH (5-(4-dimethylaminobenzoyl)-aminovaleric acid hydroxamate) to prevent dedifferentiation in cultures of primary hepatocytes is investigated. To this end, rat hepatocytes were isolated in the presence of 50μM 4-Me2N-BAVAH and exposure was subsequently continued for 7 days in monolayer culture (0...

The antibiotic albomycin is transported into cells of Escherichia coli K-12 by the same uptake system as the iron-supplying ferrichrome complex. The iron-complexing hydroxamate moieties of albomycin and ferrichrome are structurally similar. During the phase of rapid iron uptake the ‘chelators were not found in the cells. In order to understand the antibiotic activity of albomycin, it was labele...

The cleavage of gelatin to smaller molecular units by aqueous hydroxylamine or hydrazine under relatively mild conditions of temperature and pH has suggested the existence of “ester-like” or imide linkages in this protein (1). The amide bonds of gelatin were cleaved only to a minor extent and could not account for the large change in molecular weight. It is known, however, that prolonged treatm...

The human histone deacetylase isoform 6 (HDAC6) has been demonstrated to play a major role in cell motility and aggresome formation, being interesting for the treatment of multiple tumour types and neurodegenerative conditions. Currently, most HDAC inhibitors in preclinical or clinical evaluations are non-selective inhibitors, characterised by a hydroxamate zinc-binding group (ZBG) showing off-...

The mechanisms for acquisition of iron by Haemophilus influenzae and their role in pathogenesis are not known. Heme and nonheme sources of iron were evaluated for their effect on growth of type b and nontypable strains of H. influenzae in an iron-restricted, defined medium. All 13 strains acquired iron from heme, hemoglobin, hemoglobin-haptoglobin, and heme-hemopexin. Among nonheme sources of p...

The enzymatic incorporation of D-alanine into membranes of Lacfobacillus casei (ATCC 7469) is described. The activity is dependent on ATP, supernatant fraction and membrane fragments; it is enhanced by the addition of Mg2+. The incorporation is insensitive to ribonuclease. The product of this reaction is characterized as a hydroxylamine (1 M, pH 7.0, 37”) labile ester that is not extracted into...

Liquid-liquid iron(III) extraction was investigated using benzyl fatty hydroxamic acids (BFHAs) and methyl fatty hydroxamic acids (MFHAs) as chelating agents through the formation of iron(III) methyl fatty hydroxamate (Fe-MFHs) or iron(III) benzyl fatty hydroxamate (Fe-BFHs) in the organic phase. The results obtained under optimized conditions, showed that the chelating agents in hexane extract...

Angiogenesis, one of the major routes for tumor invasion and metastasis represents a rational target for therapeutic intervention. Recent development in drug discovery has highlighted the diverse biological and pharmacological properties of hydroxamate derivatives. In this study, we characterized the anti-angiogenic activities of a novel aliphatic hydroxamate, WMJ-S-001, in an effort to develop...