In previous work the existence of free thyroxine in human serum was verified by dialysis through cellophane; the concentration of the unbound hormone in normal human serum was found to approximate one-thousandth of the total thyroxine (1). This direct measurement of free thyroxine was in reasonable agreement with the theoretical value computed from data on the per cent of total thyroxine bound ...

In this solid-phase competitive enzymoimmunoassay for albumin in human urine, antiserum to human serum albumin labeled with horseradish peroxidase (EC 1.11.1.7) is incubated with solid-phase-bound human serum albumin in the presence of sample or standard. Results obtained correlate well (r = 0.96) with those of an established fluoroimmunoassay. The present assay covers the range 0.9 to 200 mg/L...

The interaction of the immobilized triazine dye Cibacron Blue 3G-A with rat, rabbit, sheep, goat, bovine and human serum albumins was studied by affinity gel electrophoresis. Dissociation constants were estimated in each instance and showed human serum albumin to have a significantly higher affinity for the dye than did albumin from any other species. Pretreatment of the defatted proteins with ...

BACKGROUND Albumin is the most abundant protein in blood plasma, and due to its ligand binding properties, serves as a circulating depot for endogenous and exogenous (e.g. drugs) compounds. Hence, the unbound drug is the pharmacologically active drug. Commercial human albumin preparations are frequently used during surgery and in critically ill patients. Recent studies have indicated that the u...

In this research, the Magnetite nanoparticles (Fe304) were prepared by coprecipitation of Fe3+ andFE solution in alkaline medium. Two kinds of surfactants, cetyl tri methyl ammonium bromide(CTAB) and cetyl pyridinum chloride (CPC) were used in the synthesis. Fe304 Nanoparticles werecoated with human serum albumin (HSA) and bovine serum albumin (BSA). Characteristics ofcoated magnetic nanopartic...

2.1.Date of QMRF: 06.10.2009 2.2.QMRF author(s) and contact details: [1]Indrek Tulp Molcode Ltd. Turu 2, Tartu, 51014, Estonia [email protected] http://www.molcode.com [2]Tarmo Tamm Molcode Ltd. Turu 2, Tartu, 51014, Estonia [email protected] http://www.molcode.com [3]Gunnar Karelson Molcode Ltd. Turu 2, Tartu, 51014, Estonia [email protected] http://www.molcode.com [4]Dimitar Dobchev Molcod...

The features of midazolam (MDZ) induced structural perturbation of human serum albumin (HSA) has been studied in detail by Fourier transformation infrared spectra (FT-IR),and circular dichroism (CD).The CD spectra was deconvoluted to quantify the changes in α helix, β sheets, β turns and random coils of the protein. UV-Vis absorption studies suggested the static type quenching procedure for thi...

The serum of a patient (L'ec) with an IgM lambda monoclonal protein was noted to bind albumin on immunoelectrophoresis. Analytical ultracentrifugation of the L'ec serum demonstrated 23S and 12S peaks, but no 4S (albumin) boundary. Immunologically identical 20S and 9S IgM proteins were isolated from the serum and the addition in vitro of either the patient's albumin or albumin isolated from norm...

Human serum albumin (HSA) is a complex protein with multiple functions and plays a key role in organ system homeostasis. Changes in albumin levels are associated with worsened outcome in critical illness. However, serum albumin functional activities cannot be easily determined to assess its capacities in illness. Two real-time and sensitive fluorescent liposome assays were developed to determin...

Approximately 10% of the albumin in normal human serum is modified by nonenzymatic glycosylation, primarily at the epsilon-amino group of lysine residue 525. Incubation of albumin with glucose under physiological conditions in vitro resulted in glycosylation of the same residue. After separation of glycosylated human serum albumin from the nonglycosylated form by boronate affinity chromatograph...