To study the specific actions of relaxin through RXFP1 in human cells, it would be advantageous to develop cell populations with permanent RXFP1 knockdown (KD). We have developed and assessed four microRNA against human RXFP1. One of the four designed microRNA displayed significant RXFP1 KD as assessed by reduced relaxin binding when co-transfected with human RXFP1 into HEK-293T cells. The sele...

Background: Mesenchymal stem cells (MSCs) are multipotent cells able to differentiating into a variety of mesenchymal tissues including osteoblasts, adipocytes and several other tissues. Objectives: Differentiation of MSCs into fibroblast cells in vitro is an attractive strategy to achieve fibroblast cell and use them for purposes such as regeneration medicine. The goal of this s...

The molecular circadian clock, which consists of transcription-translation feedback loops, is robust in all skin layers. As dysfunction associated with diseases, understanding the clock may provide insights into disease development and management. Particularly, core machinery interacts elements wound healing process such as fibroblast migration, manipulation network or alternation wounding time...

miRNAs as one of the potential therapeutic agents have been recently considered for cancer treatment. AS1411 (aptNCL) is a DNA aptamer specifically binding to nucleolin protein on the cancer cell surface with antiproliferative effect. The aim of the study was to develop a conjugate consisted of aptNCL (as targeted delivery of therapeutic agent) and miRNA let-7d (as a tumor suppressor) using two...

To improve understanding of acne we evaluated host cell networks in the pilosebaceous unit and variables associated with C. acnes that promote skin inflammation. Single-cell RNA sequencing uncovered unsuspected activation specific dermal fibroblast subsets both human mice challenged by acnes. This transcriptional response was characterized initiation fat differentiation expression cathelicidin ...

OBJECTIVE Allograft inflammatory factor 1 (AIF-1) was first identified in rat cardiac allografts undergoing chronic rejection. The vasculopathy of chronic allograft rejection is strikingly similar to that seen in patients with systemic sclerosis (SSc). We previously demonstrated AIF-1 expression in inflammatory cells infiltrating skin and lungs from SSc patients, but its role in SSc pathogenesi...