نتایج جستجو برای: human antibody library

تعداد نتایج: 1863887  

2010
Li Du Li-Jun Zhou Xiu-Jie Pan Yu-Xiao Wang Qin-Zhi Xu Zhi-Hua Yang Yu Wang Xiao-Dan Liu Mao-Xiang Zhu Ping-Kun Zhou

BACKGROUND Overexpression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is commonly occurred in cancers and causes radioresistance and poor prognosis. In present study, the single-chain variable antibody fragments (scFv) targeting DNA-PKcs was developed for the application of radiosensitization in vitro and in vivo. A humanized semisynthetic scFv library and the phage-display ant...

Journal: :Acta biochimica et biophysica Sinica 2011
Ivan Zhou Zhe-Huan Zhang Chang-Zheng Li Zhen-Rui Chen Wei He Ye Zhou Shuwen Liu Shuguang Wu Yuanping Zhou Wanlong Tan Shibo Jiang Chen Zhou

A unique four-way ligation strategy was developed for rapid construction of a full-length antibody library. A mammalian expression vector was constructed that contained dual mammalian expression cassettes and sequences recognized by the unique restriction enzymes BsmBI, BstXI, and SfiI. Both full-length light-chain and variable domain of heavy-chain genes were inserted into the vector in one st...

Erythropoietin (EPO) was first known as a factor for red blood cell proliferation and differentiation. Recent studies show the effects of EPO on immune system. In this study the antihuman leukocyte antibody (anti-HLA) titer were determined in five groups of rats, which had been sensitized with human lymphocyte. Also, the effects of stimulation's frequency and dose of recombinant human erythropo...

Journal: :Journal of the University Librarians Association of Sri Lanka 2007

2013
Matthew B. Tessier Oliver C. Grant Jamie Heimburg-Molinaro David Smith Snehal Jadey Andrew M. Gulick John Glushka Susan L. Deutscher Kate Rittenhouse-Olson Robert J. Woods

Recombinant antibodies are of profound clinical significance; yet, anti-carbohydrate antibodies are prone to undesirable cross-reactivity with structurally related-glycans. Here we introduce a new technology called Computational Carbohydrate Grafting (CCG), which enables a virtual library of glycans to be assessed for protein binding specificity, and employ it to define the scope and structural...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1998
H J Jung W Ni

A large library of phage-displayed human single-chain Fv antibodies (scFv), containing 6.7 3 109 members, was generated by improving the steps of library construction. Fourteen different protein antigens were used to affinity select antibodies from this library. A panel of specific antibodies was isolated with each antigen, and each panel contained an average of 8.7 different scFv. Measurements...

Journal: :Cancer research 1999
N Martínez A Alonso M D Moragues J Pontón J Schneider

It has been previously shown (J. Schneider et al., Br. J. Cancer, 77: 1015-1020, 1998) that an antibody directed against Candida albicans proteins (C6) recognizes specifically a protein in human ovarian tumors. We have now performed an immunoscreening of a human cDNA library using this antibody and identified the antigen as Nup88, a protein localized preferentially at the nuclear membrane and p...

2015
Chao-Ping Tung Ing-Chien Chen Chung-Ming Yu Hung-Pin Peng Jhih-Wei Jian Shiou-Hwa Ma Yu-Ching Lee Jia-Tsrong Jan An-Suei Yang

Broadly neutralizing antibodies developed from the IGHV1-69 germline gene are known to bind to the stem region of hemagglutinin in diverse influenza viruses but the sequence determinants for the antigen recognition, including neutralization potency and binding affinity, are not clearly understood. Such understanding could inform designs of synthetic antibody libraries targeting the stem epitope...

Journal: :Molecular cancer therapeutics 2009
Yang Feng Xiaodong Xiao Zhongyu Zhu Emily Streaker Mitchell Ho Ira Pastan Dimiter S Dimitrov

Mesothelin is a potential new target for cancer immunotherapy because it is present at relatively low levels only in mesothelial cells of pleura, peritoneum, and pericardium of healthy people, but is significantly elevated in a number of tumors, including mesothelioma, ovarian, pancreatic, and lung cancers. However, all currently available antibodies against mesothelin are either murine or chim...

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