Presents remarks from the MTTS Ombudsman.

Parthenolide (PT), a principal active component in medicinal plants, has been used conventionally to treat migraine and inflammation. This component has recently been reported to induce apoptosis in cancer cells, through mitochondrial dysfunction. In the present study, we investigated PT-mediated cell death signaling pathway by focusing on the involvement of Bcl-2 family members in human colore...

Diabetic nephropathy (DN) is one of the most common and serious chronic complications of diabetes mellitus, however, no efficient clinical drugs exist for the treatment of DN. We selected and synthesized several sesquiterpene lactones (SLs), and then used the MTT assay to detect rat mesangial cells (MCs) proliferation, ELISA to measure the expression level of monocyte chemoattractant protein-1 ...

In the present study, SGC-7901/DDP cells were treated with different concentrations of parthenolide (PN) (2.5-15 µmol/l), cisplatin (DDP) (1.25-15 µg/ml) and PN+DDP. The proliferation inhibition rates were measured using an MTT assay, and the synergies of PN and DDP were analyzed. The effect of PN and DDP on SGC-7901/DDP cell proliferation demonstrated a time- and concentration-dependent associ...

An HPLC-Charged Aerosol Detection (CAD) was employed to simultaneously determine saikosaponins include saikosaponin a (SSa), saikosaponin b2 (SSb2) and saikosaponin d (SSd) in Bupleurum Radix from different locations. Meanwhile, MTT assay was used to compare the proliferation effect of SSa, SS and SSb2 on mouse splenocytes, and ELISA method was carried to determined cytokines included IL-4, IL-...

RESEARCH DESIGN AND METHODSdWe performed a frequently sampled intravenous glucose tolerance test (FSIGT) withminimal model analysis and ameal tolerance test (MTT) in 20 subjects with impaired fasting glucose (11 men, 9 women; mean age 60.76 1.9 years, BMI 29.4 6 0.9 kg/m) in a single-blind study after 2 weeks of placebo treatment and 8 weeks of colesevelam 3.75 g daily. From these tests, insuli...

The main objective of this research work was to evaluate the variation in nutritional profile, antioxidant, anti-proliferative and antibacterial activities selected species Brassica. Five locally grown Brassica (cauliflower, broccoli, red cabbage, white cabbage Chinese cabbage) were collected from Ayub Agriculture Research Institute (AARI), Faisalabad, Pakistan. Polyphenolic rich extracts these...

BACKGROUND/AIMS Parthenolide (PT), a principle component derived from feverfew (Tanacetum parthenium), is a promising anticancer agent and has been shown to promote apoptotic cell death in various cancer cells. In this study, we focused on its functional role in apoptosis, migration, and invasion of human colorectal cancer (CRC) cells. METHODS SW620 cells were employed as representative human...