A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of five hydroxyanthraquinones (aloe-emodin, rhein, emodin, chrysophanol, and physcion) in Rhubarb and experimental animal bodies. A Zorbax SB-C18 column (250 mm x 4.6 mm i.d., 5 microm) and a methanol-0.5% acetic acid (85:15, v/v) mobile phase were used for the separation...

the anthocyanins of the fruit skin of iranian forest pear (sange khouj) were separated, identified and quantified using high-performance liquid chromatography/diode array detection (hplc/dad) and hplc/electrospray ionization /mass spectrometry (hplc/esi /ms). the individual anthocyanins were identified by comparing their mass spectral data and retention times with those of standards and publish...

In recent years, photodiode array detectors (DADs) have been much improved with respect to wavelength accuracy and resolution, sensitivity, linearity and software operation. UV spectra of drugs measured with up-to-date DADs from different manufacturers are in excellent agreement, have the same quality as measured by a conventional UV spectrometer and are highly reproducible. The calculation of ...

The genus Rhanterium (Asteraceae) is a widely distributed medicinal plant throughout western North Africa and some species are used in folk medicine. aim of research was to investigate methanolic extracts from different parts (flowers, leaves, stems) Tunisian suaveolens as potential sources bioactive products useful for healthy purposes. In particular, were analyzed the phenolic composition the...

Abstract: Aim: A new stability indicating RP-HPLC based methods were developed to identify the possible degradation products of Ivermectin in isolated rat hepatocytes and different pH media using a C18 RP column. Complete validation, including linearity, accuracy, recovery, precision, robustness, stability, peak purity, was performed. Materials Methods: HPLC system UltiMateDionex 3000 DAD with ...

An efficient method is provided to detect simultaneously some important veterinary drugs from different classes in highly complex animal tissue matrix. This method using matrix solid-phase dispersion (MSPD) and high performance liquid chromatography (HPLC) with diode array detection (DAD) is developed to effectively determine two fluoroquinolones (enoxacin and lomefloxacin), two sulfonamides (s...

Alismatis Rhizoma (AMR) is a well-known natural medicine with a long history in Chinese medicine and has been commonly used for treating a wide range of ailments related to dysuria, edema, nephropathy, hyperlipidaemia, diabetes, inflammation as well as tumors in clinical applications. Most beneficial effects of AMR are attributed to the presence of protostane terpenoids, the major active ingred...

In order to examine the antioxidant properties of five different extracts of Trifolium pratense L. (Leguminosae) leaves, various assays which measure free radical scavenging ability were carried out: 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide anion and nitric oxide radical scavenger capacity tests and lipid peroxidation assay. In all of the tests, only the H2O and (to some extent) the ...

Fenthion (O,O-dimethyl-O-[3-methyl-4-(methylthio)-phenyl]-thiophos-phate ) is an organophosphate insecticide. A specific method to quantitate fenthion in postmortem matrices with solid-phase extraction combined with high-performance liquid chromatography-diode-array detection (HPLC-DAD) and gas chromatography-mass spectrometry (GC-MS) is presented. Fenitrothion (O,O-dimethyl-O-[3-methyl-4-nitro...