Herfst L, Burgalossi A, Haskic K, Tukker JJ, Schmidt M, Brecht M. Friction-based stabilization of juxtacellular recordings in freely moving rats. J Neurophysiol 108: 697–707, 2012. First published April 18, 2012; doi:10.1152/jn.00910.2011.—Virtually nothing is known about the activity of morphologically identified neurons in freely moving mammals. Here we describe stabilization and positioning ...

A new voltage-clamp apparatus for the squid axon has been implemented to enable recording of currents through small areas of axon membrane. The performance of this clamp was tested by recording total sodium currents from perfused axons (I total) and sodium currents from small membrane patches (I patch), which were recorded from inside the axon with an L-shaped pipette. The I patch records, alth...

A novel type of mechanosensory mechanism is found in Chlamydomonas reinhardtii. When a cell is captured with a suction pipette and a negative pressure is applied, the cell produces repetitive Ca2+ impulses at a frequency of 0.5-1.0 Hz. The impulse frequency increases with the applied pressure. The impulses are produced when the flagella are sucked into the pipette but not when the cell body is ...

A single cell electrophysiological analysis device with a sub-micrometer opening on embedded recording electrode has been demonstrated using combined microand nano-machining process. This technique can reduce the series pipette resistance while keeping high seal resistance for lectrophysiological measurements. In the prototype demonstration, the electrolyte pipette resistance is reduced to 200 ...

BACKGROUND Gene electrotransfer is a non-viral gene delivery method that requires successful electroporation for DNA delivery into the cells. Changing the direction of the electric field during the pulse application improves the efficacy of gene delivery. In our study, we tested a pipette tip with integrated electrodes that enables changing the direction of the electric field for electroporatio...

The aim of this study was to examine the potential relationship between the occurrence of orthodontic root resorption and presence of dental anomalies such as tooth agenesis and pipette-shaped roots. Dental anomalies and root resorption were assessed on dental panoramic tomographs (DPT) of 88 subjects, 27 males and 61 females, mean age 28.4 (SD = 11.3 years), selected from orthodontic patients ...

Video techniques were used to record chemotactic responses of filamentous cells of Escherichia coli stimulated iontophoretically with aspartate. Long, nonseptate cells were produced from polyhook strains either by introducing a cell division mutation or by growth in the presence of cephalexin. Markers indicating rotation of flagellar motors were attached with anti-hook antibodies. Aspartate was...

External or internal application of proton on the calcium current was investigated using the whole-cell patch clamp technique in single ventricular cells of the guinea pig. The effect of acid pHo on the calcium current depended on the strength of the buffer in the internal solution. In the presence of 5 mM HEPES in the pipette, external acidification depressed calcium current with the half maxi...

The classical micro-pipette aspiration technique, applied for measuring the membrane bending elasticity, is in the present work reviewed and extended to span the range of pipette aspiration pressures going through the flaccid (low pressures) to tense (high pressures) membrane regime. The quality of the conventional methods for analysing data is evaluated using numerically generated data and a n...

Classically, the grain size of soil and sediment samples is determined by the sieve method for the coarse fractions and by the pipette method, based on the ‘Stokes’ sedimentation rates, for the fine fractions. Results from the two methods are compared with results from laser diffraction size analysis, which is based on the forward scattering of monochromatic coherent light. From a point of view...