نتایج جستجو برای: hoechst 33342
تعداد نتایج: 2307 فیلتر نتایج به سال:
Cross-linking of histone proteins to DNA by UV illumination of chromatin stained with Hoechst 33342.
The photochemical effects of near-UV light on chromatin labeled with the vital DNA dye Hoechst 33342 (H33342) are studied. Several types of experiments demonstrate that illumination at both 365 and 410 nm results in significant cross-linking of proteins with the DNA. Fluorescence microscopy of dye-stained Xenopus XTC-2 nuclei shows that UV illumination has effects similar to chemical fixation b...
Hematopoietic stem cells (HSC) are multipotent cells that reside in the bone marrow and replenish all adult hematopoietic lineages throughout the lifetime of the animal. While experimenting with staining of murine bone marrow cells with the vital dye, Hoechst 33342, we discovered that display of Hoechst fluorescence simultaneously at two emission wavelengths revealed a small and distinct subset...
Results: Endothelial cells exhibited high viability (>90%) as measured via confocal microscopy using Hoechst 33342 and propidium iodide staining. Following 7 days of incubation, the necessary structural integrity of the endothelial monolayer lining the lumen was achieved. This was proven by the formation of a well-defined network of actinfilaments, stained with Alexa Fluor 488 ̈ phalloidin. Addi...
New analogues of the minor groove binding ligand Hoechst 33342 have been investigated in an attempt to improve radioprotective activity. The synthesis, DNA binding, and in vitro radioprotective properties of methylproamine, the most potent derivative, are reported. Experiments with V79 cells have shown that methylproamine is approximately 100-fold more potent than the classical aminothiol radio...
In recent years, there has been an increased effort to digitise whole-slide images of cancer tissue. This opened up a range new avenues for the application deep learning in oncology. One such avenue is virtual staining, where model tasked with reproducing appearance stained tissue sections, conditioned on different, often times less expensive, input stain. However, data train models supervised ...
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