We previously determined that a linear co-polymer of histidine and lysine (HK) in combination with liposomes enhanced the transfection efficiency of cationic liposomes. In the current study, we designed a series of HK polymers with increased branching and/or histidine/lysine ratio to determine if either variable affects transfection efficiency. In the presence of liposomes, the branched polymer...

OBJECTIVE To demonstrate the effect of hypokinesia (HK) and post-HK on phosphate (Pi) imbalance and use of Pi with different Pi imbalance and different Pi consumption: were measured Pi balance, plasma Pi level and Pi loss during HK. METHODS Experiments were conducted during the pre-experimental period of 30 days, and the HK period of 364-days and post-HK period of 30-days. Forty healthy male ...

BACKGROUND The relationship between HIV knowledge and HIV-related behaviors in settings like Mozambique has been limited by a lack of rigorously validated measures. METHODS A convenience sample of women seeking prenatal care at two clinics were administered an adapted, orally-administered, 27 item HIV-knowledge scale, the HK-27. Validation analyses were stratified by survey language (Portugue...

Most organisms anticipate daily environmental variations and orchestrate cellular functions thanks to a circadian clock which entrains robustly to the day/night cycle, despite fluctuations in light intensity due to weather or seasonal variations. Marine organisms are also subjected to fluctuations in light spectral composition as their depth varies, due to differential absorption of different w...

Chronic lymphocytic leukemia (CLL) B cells have defects in apoptosis pathways and therefore accumulate in vivo. However, when removed from the patient and cultured in vitro, these malignant cells rapidly undergo apoptosis. Recent studies suggest that leukemia cell survival is influenced by interactions with nonleukemia cells in the microenvironment of lymph nodes, marrow, and other tissues. To ...

Let k be a positive integer. The limiting distribution of the nonparametric maximum likelihood estimator of a k−monotone density is given in terms of a smooth stochastic process Hk described as follows: (i) Hk is everywhere above (or below) Yk, the k− 1 fold integral of two-sided standard Brownian motion plus (k!/(2k)!)t2k when k is even (or odd). (ii) H(2k−2) k is convex. (iii) Hk touches Yk a...

In this paper, by considering the g-circulant matrix Cn,g(H) = gcirc(Hk,1,Hk,2, . . . ,Hk,n) whose entries are the generalized k-Horadam numbers, we present a new generalization to compute spectral norm, determinant and inverse of Cn,g(H). In fact the results in here are the most general statements to obtain the inverses and determinants in such matrices having the elements of all second order ...

INTRODUCTION Diagnosis of HSV-1 keratitis (HK) is frequently based on clinical findings. Invasive specimens (corneal scrapings, biopsies) are required for microbiological diagnosis. METHODS Corneal scrapings and conjunctival swabs were collected on patients with/without clinical suspicion of HK from 2007 to 2012. RESULTS The sensitivity, specificity, positive and negative predictive values ...

The effects of oligomycin on Na+-ATPase of high K (HK) and low K (LK) sheep erythrocyte membranes have been investigated. Activation of LK Na+-ATPase is observed with ATP 5 0.2 pM; activation of HK is observed with ATP 5 0.02 PM. Inhibition occurs with higher ATP. At 0.2 pM ATP, oligomycin stimulation of LK Naf-ATPase is associated with a 3to 4-fold increase in the 3ZP-“intermediate;” inhibitio...

Bradykinin (BK), generated from high-molecular-weight kininogen (HK) is the major mediator of swelling attacks in hereditary angioedema (HAE), a disease associated with C1-inhibitor deficiency. Plasma kallikrein, activated by factor XIIa, is responsible for most of HK cleavage. However other proteases, which activate during episodes of angioedema, might also contribute to BK production. The lec...