background: angiogenesis is an important process in various physiologic and pathologic states. the most significant stimulator of angiogenesis is vascular endothelial growth factor (vegf). in contrast, vasohibin1 acts as an angiogenesis inhibitor which specifically inhibits new vessels formation. the aim of the present study was cloning and expression of vasohibin1 gene in e. coli as well as pu...

We investigated the organization of the immunoglobulin heavy chain (IgH) and the T cell receptor beta chain (T beta) gene loci in 20 ocular adnexal and four extraocular lymphoid neoplasms obtained from 18 patients presenting with an ocular adnexal lymphoid neoplasm. Fifteen ocular adnexal and four extraocular lymphoid neoplasms occurring in 13 patients were classified by morphological examinati...

The presence of the translocation t(8;14)(q24;q32) has not been well described in follicular lymphoma (FL). In a consecutive series of 278 karyotypically abnormal non-Hodgkin's lymphomas (NHL), six patients with FL showing a t(8;14) without a t(14;18)(q32;q21) were identified. They ranged in age from 45 to 73 years. The cell type was mixed in four patients, small-cleaved in one, and large-cleav...

Polymorphism. PstI restriction fragment length polymorphism (RFLP) in the ovine Aconitase 2 (AC02) gene. Source and Description of Probe. The probe is a .9kb AC02 cDNA from a porcine myocardial hgt 10 library (Zheng et al., 19901, cloned into the EcoRI site of pUC118. The insert was gel-purified and radiolabeled with [ c Y ~ ~ P I ~ C T P by the random primer method to specific activity > lo9 d...

All Legionella species express a 60-kilodalton (kDa) protein which contains a genus-specific epitope recognized by murine monoclonal antibody GW2X4B8B2H6. A genomic cosmid library of Legionella pneumophila chromosomal DNA was constructed in pHC79 and screened for 60-kDa antigen-expressing clones with the monoclonal antibody. A 3.2-kilobase EcoRI fragment from cosmid 14B11 expressing a 60-kDa pr...

DNA marker is commonly used to determine the size of fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure prepare recombinant plasmids pSY-60 which was partially digested one restriction enzyme for generating markers 7 from 60 420 bp. The included synthesis bp fragment with EcoRI sites at both ends using PCR extension, self-ligation an...

seven geographic isolates of helicoverpa armigera npv were characterized by pcr-rflp techniques and their comparative virulence to second instar larvae of h. armigera (hã¼bner) were compared before and after exposure to simulated sunlight as well as open weather conditions on cotton and tomato plants. the pcr products of 25k fp region showed no difference among the isolates (780 bp). the pcr am...

elaboration of different toxins by enterotoxigenic e. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. various strategies have been employed to raise antibodies against these toxins as a line of defense. in this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...

Methods Wild type human von Willebrand factor cDNA: Von Willebrand factor cDNA was cloned from the Marathon-ready human bone marrow cDNA library (Clontech, Mountain View, CA) using the following three sets of primers: 5'-CCATCCTAATACGACTCACTATAGGGC-3’ / 5'TCAATCTCTCCTCCCTCCACCAGGAT-3' (VWF 3-3103), 5'CTCACCTTCGACGGGCTCAAATACCTG-3' / 5'-TGGGAGCTGTCGGGGACAAGACAC-3' (VWF 2925-6712), 5'-AGCGAATCGAA...