We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain ...

Pulmonary infections are common in human immunodeficiency virus (HIV) positive persons. Pneumoystis carinii (PC), now named as pneumocystis jiroveci, is the most frequently encountered pathogen. The study, carried out from June 1999 to May 2005, comprised as many as 1101 HIV positive persons presumed to be suffering from pneumocystis carinii pneumonia. Induced sputum samples were obtained from ...

quebracho tannin wood adhesive in non formaldehyde approach has low autocondensation rate and needs more press time than formaldehyde-based wood adhesives to produce wood composites. in this study, boric acid was used to increase autocondensation rate of quebracho tannin adhesive to make poplar plywood. the main ingredients of the adhesives include quebracho tannin, naoh, hexamine, boric acid a...

Methenamine silver stain was used to compare the number of cysts of Pneumocystis carinii contained in lung concentrate smears of homogenized lung tissue with the number in impression smears. Results were also compared with histopathological examination of methenamine silver-stained paraffin-embedded sections. Of slides from 23 preparations, a greater number of cysts were contained in concentrat...

BACKGROUND There is limited data on comparing stains in the detection of microsporidia in corneal biopsies. Hence we wanted to evaluate various stains for their ability to detect microsporidia in corneal tissue sections. METHODS Four cases diagnosed with microsporidiosis on Hematoxylin and Eosin and Periodic Acid Schiff's stained sections of the corneal button between January 2002 and Decembe...

INTRODUCTION The opportunistic fungus Pneumocystis jirovecii causes Pneumocystis pneumonia (PcP), which is a life-threatening infection in HIV/AIDS patients. The seemingly low prevalence of P. jirovecii pneumonia in sub-Saharan Africa has been a matter of great debate because many HIV/AIDS patients reside in this region. The lack of suitable diagnostic practices in this resource limited-region ...

The presence of different immunoglobulins (lgG, IgM, and IgA) was studied in 50 routinely fixed paraffin embedded renal biopsies. lgG deposits was seen independently in 26 (52%) cases, in combination with IgA in 14 (28%) cases, with IgM in 1 (2%) case and with both lgA/lgM in 1 (2%) case. lgA alone was seen in 1 (2%) case only. IgM was seen only in 2 (4%) cases. In one case it was seen in combi...

Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis carinii pneumonia, it does not normally stain the cyst wall. For detection of the cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining w...

The technique of immunoblotting for detecting soluble Pneumocystis carinii antigen(s) in bronchoalveolar lavage fluid specimens from patients with AIDS and Pneumocystis pneumonia was evaluated. A soluble 67 kilodalton polypeptide that was immunoreactive with an anti-P carinii monoclonal antibody (2G2) was found in the supernatants of 26 lavage samples from patients with pneumocystosis. Intact o...