Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 'apo', CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols d...

Increased knowledge of the mechanisms that determine respiratory failure has led to the development of new technologies aimed at improving ventilatory treatment. Proportional assist ventilation and neurally adjusted ventilatory assist have been designed with the goal of improving patient-ventilator interaction by matching the ventilator support with the neural output of the respiratory centers....

Because nonalcoholic steatohepatitis can progress towards cirrhosis even in children, early detection of hepatic fibrosis and accurate diagnosis of nonalcoholic fatty liver disease (NAFLD) are important. Although liver biopsy is regarded as the gold standard of diagnosis, its clinical application is somewhat limited in children due to its invasiveness. Noninvasive diagnostic methods, including ...

introduction: cardiovascular diseases are important factors at human death and factor destroys human resources. health intervention is a service for developing of health and diagnoses or modifies of process diseases. it is necessary that every country regarding to its goals, laws national, interests and standards, considers establishing such a system. a comparison of cardiovascular disease clas...

Assisted ventilation is a highly complex process that requires an intimate interaction between the ventilator and the patient. The complexity of this form of ventilation is frequently underappreciated by the bedside clinician. In assisted mechanical ventilation, regardless of the specific mode, the ventilator's gas delivery pattern and the patient's breathing pattern must match near perfectly o...

background all conditions that interfere with blood flow at any level within the portal system can lead to portal hypertension. for better management of this disorder, it is important to determine the underlying cause. in previ­ous studies, extra-hepatic disorders have been reported as the main cause of portal hypertension in children. in this study, we investigate the under­lying causes of por...

conclusions capillaria hepatica should be considered in differential diagnoses in any child with fever, hepatomegaly, eosinophilia and hyperglobulinemia. case presentation the current paper reports a case of capillaria hepatica infection in a four-year-old boy which presented with fever, hepatomegaly, peripheral eosinophilia, and noticeable weight loss. the diagnosis was made on the histologica...