mycoplasma agalactia was cultured in pplo broth medium supplemented with sterilized normal horse serum in fermentor. cultivation process continued at 37 ºc under aerobic conditions and growth was terminated after 26 hours cultured period with 2.2×109 mycoplasma per ml. samples were taken at different stages of growth. culture was inactivated with 0.04 %v/v formaldehyde and the vaccine was adjuv...

In this study, susceptibilities were determined for AZD0914, a spiropyrimidinetrione DNA gyrase inhibitor, azithromycin, doxycycline, and levofloxacin against Mycoplasma and Ureaplasma species. The activity of AZD0914 was comparable to that of levofloxacin and doxycycline against Mycoplasma genitalium and Mycoplasma pneumoniae. The AZD0914 MIC90 against Mycoplasma hominis was 8-fold greater tha...

Objective: We examined the blood of 48 North American Chronic Fatigue Syndrome (CFS) patients subsequently diagnosed with Lyme Disease Borrelia burgdorferi and compared these to 50 North American CFS patients without evidence of Borrelia burgdorferi infections for presence of Mycoplasma spp. co-infections using forensic polymerase chain reaction. Results: We found that 68.75% of CFS/Lyme patien...

3. Baseman JB, Tully JG. Mycoplasma: sophisticated, reemerging, and burdened by their notoriety. Emerg Infect Dis. 1997;3:21–32. 4. Baker AS, Ruoff KL, Madoff S. Isolation of Mycoplasma species from a patient with seal fi nger. Clin Infect Dis. 1998;27:1168– 70. DOI: 10.1086/514980 5. Lierz M, Hagen N, Harcourt-Brown N, Hernandez-Divers SJ, Lueschow D, Hafez HM. Prevalence of mycoplasmas in egg...

BACKGROUND In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for the detection in bronchoalveolar lavage fluid (BALF) of Mycoplasma (M.) dispar, M. bovis and M. bovirhinis, all three associated with bovine respiratory disease (BRD). Primers and probes of the RespoCheck Mycoplasma triplex real-time PCR are based on the V3/V4 region of the 16S rRNA gene of the three Mycop...

Firmino F.P., Aquino L.C., Marçola T.G., Bittencourt M.V., McManus C.M. & Paludo G.R. 2016. Frequency and hematological alterations of different hemoplasma infections with retrovirusis co-infections in domestic cats from Brazil. Pesquisa Veterinária Brasileira 36(8):731-736. Laboratório de Patologia Clínica, Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Campus Univers...

Prokaryotic organisms of the genus Mycoplasma are characterized by their small body and genome size containing a 0.6-1.35 M bp genome. The genome is noted for its low G+C frequency ranging from 8-40 mol%. The Mycoplasma genus stems from the class Mollicutes (for soft skin), which lacks the cell walls and external motility appendages often present in other bacteria. To date, there are more than ...

Mycoplasma infection has been reported to be associated with cancer migration, invasion, epithelial-mesenchymal transition as well as the resistance to nucleoside analogues chemotherapeutic drugs. In this study, we found that the sensitivity of hepatocarcinoma cells to Cisplatin, Gemcitabine and Mitoxantrone was increased by mycoplasma elimination. Similar to the effect of anti-mycoplasma agent...