conclusions our underlying hypothesis is that recapitulating the three-dimensional early embryonic structure, in order to reproduce better in vitro the three-dimensional morphogenetic-like re-arrangements, would improve cells differentiation, when in vivo transplanted; moreover, it could be used as a simplified cancer disease model and reliable drug evaluation method as well. results the proces...

The fatty acid (FA) composition of cell membranes represents a metabolic biomarker. However, the FA profile reproducibility in cultures remains significant challenge. In this study, ratios are validated as markers alternative to FA. To goal, samples belonging cancer HeLa cells and normal 3T3 fibroblasts, from various experimental sets, analyzed by high-performance liquid chromatography system c...

Chronic thermotolerance is an operational definition for that resistance to cell killing by heat which develops during a protracted exposure at temperatures generally in the range of 41.5-42.5 degrees C which is usually observed as a reduction in the slope of the survival curve. While Chinese hamster ovary (CHO) cells are generally more sensitive to high-temperature heat shock than HeLa cells, ...

Introduction: The human immunodeficiency virus (HIV) is a threat to global health and the need for finding new methods of antivirus research, in particular against HIV has increased in recent years. In this study, we investigated the ability of the cold atmospheric pressure plasma jet (CAPPJ) using helium to inhibit the replication of HIV virions. Methods: Single cycle replicable HIV (SCR-HIV) ...

The cell surface aspects and the density, distribution and morphology of surface cytoplasmic projections (microprocesses) of HeLa, BHK and baby mouse kidney cells, maintained under different culture conditions, have been compared using scanning electron microscopy. No significant differences were noted in the surface aspects of these cell strains. Comparison of the 3 cell strains, under identic...

There are a sub-population of cells in tumor tissues known as cancer stem cells (CSCs) which have similar features with stem cells, including self-renewal and differentiation capacity. Recently, it was established that not only stem cells factors such as Oct4, but also ES-associated lncRNAs are contributing to various regulatory aspects of CSCs. Myocardial infarction associated transcript (MIAT...

Chronic thermotolerance is an operational definition for that resistance to cell killing by heat which develops during a protracted exposure at temperatures generally in the range of 4I.5-42.5T which is usually observed as a reduction in the slope of the survival curve. While Chinese hamster ovary (CHO) cells are generally more sensitive to high-temper ature heat shock than HeLa cells, studies ...

The techniques previously available for the study of purified animal viruses have not permitted the production under controlled conditions of sufficient virus for experiments involving degradative chemical analysis. The utilization of suspension cell cultures (1) and the development of a simple, convenient virus purification procedure (2) have now made possible an investigation of the questions...

This study aims to determine the condition factors influencing the in vitro proliferation culture of spermatogonial stem cells (SSCs) through isolation and culture of SSCs. With mouse embryonic fibroblast (MEF) cells, SIM-6-thiogunanie-oualiain (STO) cells and Hela cells used as the feeder layers, we compared the numbers of cell clones treated with mitomycin C as well as the proliferation condi...

We used the fluorescence ubiquitination-based cell cycle indicator (FUCCI) to monitor cell cycle arrest after treatment of FUCCI-expressing HeLa cells (FUCCI-HeLa) with a traditional Chinese medicine (TCM) herbal mixture LQ, previously shown to have anti-tumor and anti-metastatic activity in mouse models. Paclitaxel was used as the positive control. In 2D monolayer culture, the untreated contro...