A total of 3,257 samples of diseased rainbow trout were examined for the presence of viruses from January 1983 to December 1987. A virus closely related to the VR 299 serotype of infectious pancreatic necrosis virus was isolated from 13 cases. An additional 7,228 viscera samples from asymptomatic fish were collected during the same period and a similar virus was isolated from 2 sites. During th...

The in vitro influence of methisoprinol on the replication of viral haemorrhagic septicaemia (VHS) and infectious haematopoetic necrosis (IHN) viruses by measuring their RNA synthesis was examined. EPC cell cultures containing various concentrations of methisoprinol (0, 100, 200, 300, 400 and 500 μg/ml of medium) were inoculated with VHS or IHN virus suspension containing 10 TCID50/ml. The inhi...

Pasteurella multocida (Robert type-1) was grown on tryptose sucrose yeast (TSY) broth in a fermenter. A sterilised air was provided that potentiated the density of the culture (5 x 10 organisms/ml). The culture was inactivated with formalin (0.5% ). The alum precipitated and oil based haemorrhagic septicaemia vaccines (APHSV and OBHSV) were prepared and their efficacies were evaluated in rabbit...

Viral haemorrhagic septicaemia virus (VHSV) genotype IVb was recently detected as the cause of numerous mortality events in Great Lakes fish. In situ hybridization was used to examine the gonads from 13 fish, including freshwater drum Aplodinotus grunniens and muskellunge Esox masquinongy that were infected naturally, as well as rainbow trout Oncorhynchus mykiss and fathead minnows Pimphales pr...

The recent introduction of the Heptavalent-pneumococcal vaccine (PCV-7) by private pharmaceutical companies in Nigeria, has generated interest in invasive bacterial diseases particularly IPD. Our objective in this study is to investigate the trend and occurrence rate of IPD in Abeokuta, Nigeria. Suspected IPD cases were assessed from Jan 2010 to Dec 2010 for demographic and Microbiological char...

The dengue viruses are estimated to cause several hundred thousand cases of dengue fever, dengue haemorrhagic fever and dengue shock syndrome annually. Attempts to prevent the infection focus on the development of a vaccine that would protect against all four serotypes of the dengue virus. Various biotechnological approaches are being explored, including the use of live attenuated or inactivate...