Molecular hybridisation of four bioactive fragments piperazine, substituted-benzofuran, amino acids, and 2,4-dinitrobenzenesulfonamide as single molecular architecture was designed. A series new hybrids were synthesised subjected to evaluation for their inhibitory activity against Mycobacterium tuberculosis (Mtb) H37Rv. 4d-f 4o found exhibit MIC 1.56 µg/mL, equally active ethambutol whereas 4a,...

A new rapid microdilution method, employing the dye resazurin as an indicator of mycobacterial growth, was developed to evaluate drug susceptibility of Mycobacterium tuberculosis reference strain H37Rv and of 13 M. tuberculosis susceptible or multidrug-resistant clinical strains. Different growth conditions were evaluated. The MICs of isoniazid, rifampicin, streptomycin and ethambutol were dete...

In the present research, we tend to ready a series of novel pyrimidine-linked isoxazole derivatives (11-20). The molecular structure and elemental composition these compounds were confirmed by spectroscopic studies analysis. MABA (Microplate alamar Blue Assay) assay was employed for assessing antitubercular activity against Mycobacterium tuberculosis H37Rv strain. Among ten synthesized compound...

This variant, designated H37Ra, is morphologically distinguishable from H37Rv. The celLs of H37Rv grow parallel to each other, leading to cord-like formations and flat colonies. The cells of H37Ra, however, are randomly clumped, yielding colonies which are higher and denser than those of H37Rv. No "cord factor" can be extracted from cultures of H37Ra (Bloch, J. Exptl. Med., 91, 197-218, 1950). ...

UNLABELLED The Mycobacterium tuberculosis Proteome Comparison Database (MTB-PCDB) is an online database providing integrated access to proteome sequence comparison data for five strains of Mycobacterium tuberculosis (H37Rv, H37Ra, CDC 1551, F11 and KZN 1435) sequenced completely so far. MTB-PCDB currently hosts 40252 protein sequence comparison data obtained through inter-strain proteome compar...

mycobacterium tuberculosis (h37rv strain) was used in this study. the bacterial cells were disintegrated by sonication. the separation and characterization of the soluble molecules were attempted by various techniques including gel filtration, ion exchange chromatographies and polyacrylamide gel electrophoresis, using sds and 2me. eight protein molecules with molecular weights ranging from 6.3 ...

After intravenous injection into rats, both the attenuated strain R1Rv and the virulent strain H37Rv of Mycobacterium tuberculosis grow in the liver and spleen. However, the infected rats mount a specific immune response with great rapidity, giving a false impression of natural resistance to the tubercle bacillus. Adoptive immunity to tuberculosis was achieved by transferring thoracic duct cell...

A series of sixteen pyrazinamide analogues with the -CONH- linker connecting the pyrazine and benzene rings was synthesized by the condensation of chlorides of substituted pyrazinecarboxylic acids with ring-substituted (chlorine) anilines. The prepared compounds were characterized and evaluated for their antimycobacterial and antifungal activity, and for their ability to inhibit photosynthetic ...