The genetic basis of modulation by dietary sucrose of the enzyme activities glucose 6-phosphate dehydrogenase (GGPD) and 6-phosphogluconate dehydrogenase (6PGD) activities in third instar larvae of Drosophila melanogaster was investigated, using isogenic lines derived from wild populations. Considerable genetically determined variation in response was detected among lines that differed only in ...

The angiogenic factor thymidine phosphorylase (TP) is highly expressed in human monocytes and macrophages, and its expression has been linked to the pathology and progression of solid tumors, rheumatoid arthritis, and gastric ulcers. In this study, TP mRNA and enzyme activity were found to be upregulated upon the induction of differentiation of the human monocyte cell line THP-1 by phorbol 12-m...

Within-laboratory collaborative projects wherein each worker performs the technique he or she does best are common nowadays. Opportunities for projects wherein everyone does everything are less common, but these projects bring their own satisfactions; the Great Translocation Hunt is presented as an example. LARGE laboratories with graduate students and postdoctoral fellows working on interwoven...

Maternal expression of the Toll gene is required for the production and the correct spatial organization of all lateral and ventral structures of the Drosophila embryo. We show here that the Toll gene is transcribed zygotically in the embryo and that zygotic expression is important for the viability of the larva. Both genetic and molecular data indicate that the zygotic Toll product has the sam...

The mechanism underlying transinactivation associated with dominant position effect variegation (PEV) of the Drosophila melanogaster brown gene has been addressed by a comparison with its D. uinlis homologue. This comparison revealed 86% identity between conceptual translation products of the h o r n gene from these two species, functional homology, as the D. virilis gene rescues a D. melanogas...

On the basis of interallelic recombination frequencies measured in diallelic crosses of the 5 amylose-extender alleles in maize, ae, B I , B3, M2 and i1, it was possible to construct a unique linear genetic map ordering all 5 alleles within the locus. The reciprocal diallelic crosses each gave comparable frequency estimates. The relative order is ae, i l , B3, BY, A42 or the reverse. Even thoug...

The Antp locus is involved in the development of the thorax of the larval and adult Drosophila. The absence of Antp' function during embryogenesis results in the larval mesothorax exhibiting characteristics of the prothorax and an ensuing lethality; the loss of Antp+ function in the development of the adult thorax causes specific portions of the leg, wing and humeral imaginal discs to develop a...

Knowledge of multigenic family organization should provide insight into their mode of evolution. Accordingly, we characterized the 5s ribosomal gene family in the Drosophila melanogaster strain $06. The 5s genes in this strain display a striking HindIII restriction difference compared to the “standard” D. mehogaster 5s genes. The sequence of three q?06 5s genes was determined. We show that the ...

A set of 1,000 “mutation accumulation” lines of Drosophila melanogaster, which originated from two different wild-type, lethal-bearing second chromosomes (YAMAGUCHI and MUKAI 1974; MUKAI and COCKERHAM 1977), was examined for evidence of a mutator factor by using the occurrence of recessive visible mutations and male recombination to identify its presence. The 1,000 lines were screened at approx...

A single genetically marked P element can be efficiently mobilized to insertionally mutagenize the Drosophila genome. We have investigated how the structure of the starting element and its location along the X chromosome influenced the rate and location of mutations recovered. The structure of two P[rosy+] elements strongly affected mobilization by the autonomous 'Jumpstarter-1" element. Their ...