In studies using isolated ovarian granulosa and thecal cells it is important to assess the degree of cross contamination. Marker genes commonly used for granulosa cells include FSHR, CYP19A1 and AMH while CYP17A1 and INSL3 are used for thecal cells. To increase the number of marker genes available we compared expression microarray data from isolated theca interna with that from granulosa cells ...

Zearalenone (ZEA) is a natural contaminant of various food and feed products representing a significant problem worldwide. Since the occurrence of ZEA in grains and feeds is frequent, the present study was carried out to evaluate the possible effects of ZEA on steroid production and gene expression of porcine granulosa cells, using RNA-seq analysis. Porcine granulosa cells were administered 10 ...

The involvement of FSH and triiodothyronine (T(3)) in circadian clocks was investigated using immature granulosa cells of ovaries during the progress of cell maturation. Granulosa cells were prepared from preantral follicles of mouse Period2 (Per2)-dLuc reporter gene transgenic rats injected subcutaneously with the synthetic nonsteroidal estrogen diethylstilbestrol. Analysis of the cellular clo...

We compare Temporal Difference Learning (TDL) with Coevolutionary Learning (CEL) on Othello. Apart from using three popular single-criteria performance measures: i) generalization performance or expected utility, ii) average results against a hand-crafted heuristic and iii) result in a head to head match, we compare the algorithms using performance profiles. This multi-criteria performance meas...

The goal of the study reported in the paper was to determine whether rendering style (non-photorealistic versus realistic) has an effect on perception of American Sign Language (ASL) finger spelling animations. Sixty-nine (69) subjects participated in the experiment; all subjects were ASL users. The participants were asked to watch forty (40) sign language animation clips representing twenty (2...

The discovery of displaced granulosa cells in the fallopian tube is concerning for a granulosa cell neoplasm and can lead to further surgical intervention. Discerning the risk of neoplasm can be challenging, even with immunohistochemical staining of CD56, a highly sensitive marker of granulosa cell neoplasm. A 48-year-old underwent an uncomplicated hysterectomy and bilateral salpingectomy for s...

Low progesterone levels are associated with luteal phase deficiency in women with polycystic ovary syndrome (PCOS). The mechanisms regulating progesterone biosynthesis in the granulosa cells from women with PCOS is largely unknown. Fractalkine is expressed in human ovaries, and is reported to regulate progesterone production in granulosa cells of healthy women. In the current study, we aimed to...

Granulosa cells play an important part in the nutrition of the oocyte during oogenesis (Raven, 1961). Anderson & Beams (1960) suggested that, in the primary follicle, nutritive material synthesized by the granulosa cells reaches the oocyte by diffusion across the zona pellucida. In the rabbit, follicle formation and elaboration of the zona pellucida begin at the end of the second week after bir...