Sea urchin is one of the marine biotas that produce bioactive compounds and has biological activity, which antibacterial. This study aims to determine antibacterial activity crude extract D. setosum gonad its fractionation against test bacteria Staphylococcus aureus Escherichia coli conduct a zoochemical analysis content compounds. The used disc diffusion method while chemical was carried out q...

Ubiquitin C-terminal hydrolases (UCHLs) are a subset of deubiquitinating enzymes, and are involved in numerous physiological processes. However, the role of UCHLs during gonad development has not been studied in crustaceans. In this study, we have first cloned and analyzed expression profiling of Sp-uchl3 and Sp-uchl5 genes from mud crab Scylla paramamosain. The full-length cDNA of Sp-uchl3 is ...

Disorders of sex development often arise from anomalies in the molecular or cellular networks that guide the differentiation of the embryonic gonad into either a testis or an ovary, two functionally distinct organs. The activation of the Y-linked gene Sry (sex-determining region Y) and its downstream target Sox9 (Sry box-containing gene 9) triggers testis differentiation by stimulating the diff...

The pearl oyster, “Pinctada radiate”, is a native species of Persian Gulf area, which has always been attractive for its ability of producing pearl. Thus, studying the gonadal maturation of the pearl oyster has been important for nucleus implantation to pearl production. In this study, samples were taken in winter and spring from Lavan and Kish Islands. The classic histological methods were use...

Microinjection is considered the gold standard technique for delivery of nucleic acids (NAs; transgenes or genome editing tools such as CRISPR/Cas9 systems) into embryos, for creating genetically modified organisms. It requires sophisticated equipment as well as well-trained and highly skilled personnel to perform the micro-injection technique. Here, we describe a novel and simple microinjectio...

The gonads of 107 spontaneous, chromosomal mosaic, day-15 fetal hermaphrodites derived from BALB/cWt strain male mice are described and photographs of seven gonads representative of the major types are presented. There were 16 ovaries, 15 testes, and 183 ovotestes. The ovotestes contained on the average more testicular tissue than ovarian, and the ovarian tissue was more frequently located at t...

The reproductive strategies of the sea urchin, Paracentrotus lividus, was studied in the Bay of Tunis. Samples were collected monthly, from September 1993 to August 1995, in two sites which differ in their marine vegetation and their exposure to wave action. Histological examination demonstrated a cycle of gametogenesis with six reproductive stages and a main breeding period occurring between A...