The objective of our study was to demonstrate the existence of a repetitive region in the goat MUC1 gene and to develop a polymerase chain reaction (PCR) protocol to analyze its polymorphism in different breeds. Using 2 primers derived from the bovine MUC1 sequence, a PCR fragment was obtained and cloned. The sequence analysis shows that the repetitive region of goat MUC1 is an array of 60 bp r...

conclusions this study demonstrated the specificity and sensitivity of the pcr-sscp method for finding mutations in the gyra gene. due to the sensitivity of most isolates to ciprofloxacin, this antibiotic should be considered an appropriate drug for the treatment of related diseases. background during the past several years, nontuberculous mycobacteria (ntm) have been reported as some of the mo...

conclusions we concluded from the results that the frequency of emb-resistant m. tuberculosis cases in iran is lower than that of many other regions. the pcr-sscp technique can separate resistant isolates from sensitive isolates. the sequencing results of this study showed mutation in codons 309 and 299 of the embb gene. in none of the resistant isolates, mutation was observed in codon 306. fur...

In Europe the Borrelia burgdorferi sensu lato complex is represented by five distinct genospecies: Borrelia burgdorferi sensu stricto, Borrelia afzelii, Borrelia garinii, Borrelia valaisiana, and Borrelia lusitaniae. These taxonomic entities are known to differ in their specific associations with vertebrate hosts and to provoke distinct clinical manifestations in human patients. However, except...

lactoferrin is a glycoprotein with molecular weight 80 kda iron-binding bond, which is composed of 690 amino acids. in most mammalian body fluids such as sweat, semen, tears, and saliva and milk neutrophil granules there. bovine lactoferrin gene be associated with susceptibility/resistance to mastitis and even with some economically important production traits. this study was carried out to det...