We present two strategies for microspotting 10 x 12 arrays of double-stranded DNAs (dsDNAs) onto a gold-coated glass slide for high-throughput studies of protein-DNA interactions by surface plasmon resonance (SPR) microscopy. Both methods use streptavidin (SA) as a linker layer between a biotin-containing mixed self-assembled monolayer (SAM) and biotinylated dsDNAs to produce arrays with high p...

Chemical surface modification methods were investigated to activate surface of poly-(dimethylsiloxane) (PDMS) for probe immobilization in enzyme-linked immunosorbent assay (ELISA). The investigations started from dramatization of PDMS surface with (3-aminopropyl)-triethoxysilane (APTES). Amino groups generated by APTES were either derived into carboxyl group by Succinic acid anhydride (SAA), th...

in this paper, the structural properties of c20 and c12b8 fullerene interacting with glycine based onthree active sites of glycine and one c atom or one b atom in c12b8 were analyzed through thedensity functional theory. it was found out that the binding of glycine to c12b8 generated a complex.our results were extremely relevant in order to identify the potential applications of functionalizedc...

low phosphorous (p) availability in soils limits production of soybean [glycine max (l.) merr.] around the world. this study was conducted to determine whether exogenous expression of the rice (oryza sativa l.) phosphates transporter gene ospt2 would increase inorganic phosphates (pi) acquisition and improve yield in transgenic soybean. cotyledonary-node explants of the soybean were inoculated ...

Guava is one of the most important commercial fruit crop in Bangladesh due to its high nutrient value, good taste and high health benefits. This study was conducted to investigate the flowering, fruiting behavior and nutritional quality of guava genotype suitable for coastal region of Bangladesh. Fifteen genotypes (PG 01 to PG 15) having satisfactory growth were selected for this study. One bra...