Surgical replacement with artificial devices has revolutionised the care of patients with severe valvular diseases. Mechanical valves are very durable, but require long-term anticoagulation. Bioprosthetic heart valves (BHVs), devices manufactured from glutaraldehyde-fixed animal tissues, do not need long-term anticoagulation, but their long-term durability is limited to 15 - 20 years, mainly be...

Recent work has cast doubt on the validity of results obtained from glutaraldehyde-fixed material in radioautography. Peters and Ashley (3) have demonstrated that glutaraldehyde binds free leucine directly onto cells of rat liver. They indicate that this bound leucine might be confused with that incorporated by protein synthesis. As an alternative fixative for radioautography, they recommend fr...

Improvement in the water stability and other related functional properties of thin (<50 μm) kafirin protein films was investigated. Thin conventional kafirin films and kafirin microparticle films were prepared by casting in acetic acid solution. Thin kafirin films cast from microparticles were more stable in water than conventional cast kafirin films. Treatment of kafirin microparticles with he...

Glutaraldehyde fixation is a tissue preservation process used in the manufacture of bioprosthetic heart valves. The objective of fixation is to produce stable, durable, and biologically inert tissue that is suitable for implantation. Collagen, a principle tissue protein, provides strength and resistance to breaking stresses in tissue and plays a critical role in its long-term durability. In 198...

The purpose of this study was to investigate the in vitro effect of two irrigation solutions used in endodontics (5.25% sodium hypochlorite and 1% glutaraldehyde) on substrate adherence capacity of macrophages to determine if these substances can alter macrophage function. Inflammatory macrophages were obtained from Wistar rats and resuspended in RPMI-1640 medium. Substrate adherence capacity a...

Eggs of the purple sea-urchin, Strongylocentrotus purpuratus, were fertilized and fixed with 2% glutaraldehyde at various stages during cortical granule exocytosis. Fixation resulted in membrane blebs being formed precisely at the point of incipient granule fusion. These blebs pinched off to form the membranous vesicles frequently seen in exocytic pockets and in the perivitelline space. In cont...