The approximate association constants of the plasma vitamin D binding globulin (Gc-globulin) for 25-hydroxycholecalciferol (25(OH)D3) and the plasma 25(OH)D3 binding capacities were measured in samples from 123 patients with a variety of disorders. No gross differences in binding affinities were observed between different groups of patients and controls. Many patients, however, had moderately r...

Gamma globulin was demonstrated by immunocytochemical fluorescence technique in many reticuloendothelial cells of the hepatic sinusoids and of the fibrous tracts in various forms of hepatitis and in postnecrotic cirrhosis. In other liver diseases and in normal livers, even in the presence of hypergammaglobulinemia, few if any gamma globulin-containing cells were found. In contrast, spleen and l...

BALB/c mice were injected with cyclophosphamide (Cytoxan) (180-280 mg/kg) i.p., and with 3 X 10T (BALB/c 9 X DBA a ) Fj (CDF) spleen cells i.v. The recipients were bled periodically and their sera tested for DBA y-globulin. All mice had levels of donor y-globulin at 2 weeks; 17/24 still had detectable levels at 6 months. A greater proportion of mice had detectable donor y-globulin for a longer ...

The fractional rates of catabolism of isotopically labeled mouse, human, bovine, and guinea pig gamma-globulins and human serum albumin were determined in mice and in guinea pigs whose serum gamma-globulin and serum albumin levels were elevated by immunization or by injections of exogenous serum proteins. These serum proteins were also followed in mice with different serum gamma-globulin levels...

This experiment was conducted to determine the blood chemistry and carcass characteristics of broilers fed malted sorghum sprouts (MSP) or wheat offal (W/O)-based diets supplemented with yeast culture enzyme. A total two hundred forty 28-day old, Marshal Broiler chickens used for were randomly allotted 8 dietary treatment groups 30 birds each. Each group replicated thrice 10 per replicate. Data...

We performed a randomized, controlled trial of intravenous immune globulin (respiratory syncytial virus [RSV] neutralizing [Nt] antibody titer of 1:950 in 5% solution) to evaluate protection against RSV-induced disease over two respiratory virus seasons. Forty-nine children (mean age at enrollment, 4.5 months) with severe congenital heart disease or bronchopulmonary dysplasia were randomized as...

Cells from leukemia L1210 and a subline resistant to methylglyoxal-bis(guanylhydrazone) elicited a demonstrable humoral antibody response in DBA/2Ha-DD mice. Using paired-label 7-globulin mixtures containing '3 ' I-labeled normal and '2 5I-labeled immune 7-globulin, we showed that the specific uptake of antiresistant L1210 cell 7-globulin by resistant L1210 cells was consistently higher than th...

A double-antibody enzyme immunoassay was developed for determination of thyroxin-binding globulin in dried blood samples on filter paper. The measurable concentration range of thyroxin-binding globulin in two 3-mm blood discs was 3.3 to 52 mg/L equivalent of serum (i.e., equivalent to the concentrations in known serum standards). Thyroxin-binding globulin in dried blood samples on filter paper ...