People can survive several days without food, but just a few days without water. People buy bottled water for a variety of reasons, including convenience, fashion, and taste or because they think it is safer than tap water. The taste of the water has to do with the way it is treated and the quality of its source, including its natural mineral content. However, taste does not always indicate saf...

Cryptosporidium parvum and Giardia lamblia are protozoa capable of causing gastrointestinal diseases. Currently, these organisms are identified using immunofluorescent antibody (IFA)-based microscopy, and identification requires trained individuals for final confirmation. Since artificial neural networks (ANN) can provide an automated means of identification, thereby reducing human errors relat...

The highly prevalent protozoan Giardia lamblia is an enteropathogen that can be asymptomatic in some individuals, while leading to persistent diarrhea and substantial morbidity in others. In this issue of the JCI, Bartelt et al. describe a mouse model of the disease and investigate the contribution of coincident malnutrition with the development of symptomatic infection. This work in part expla...

Clinical isolates from protozoan parasites such as Giardia lamblia are at present practically impossible to culture. By using simple cyst purification methods, we show that Giardia whole genome sequencing of clinical stool samples is possible. Immunomagnetic separation after sucrose gradient flotation gave superior results compared to sucrose gradient flotation alone. The method enables detaile...

Sir,?I trust that Surgeon-Lieutenant-Colonel Moran will not think that I desired to ignore his work on the above subject in any ommiinication with you, whether designed for formal publication or otherwise. I take it, however, that every one interested in the subject is well aware of the fact of Dr. Moran's discovery of the prevalence of the disease in the Gorakhpur district, originally publishe...

OBJECTIVES This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of Cryptospofidium parvum and Giardia lamblia in patients with diarrhea. METHODS The cultivated C. parvum oocysts and G. lamblia cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were sepa...

background : the aim of the present study was to evaluate the effects of metronidazole and albendazole against clinical isolates of giardia lamblia in vitro. methods : from all human samples of containing cysts, 10 isolates were successfully excysted in vitro . trophozites viability was assessed by eosine 0.1% and cultured axenically in tyi-s-33 modified medium supplemented with heat inactivate...

Giardiasis is one of the most frequent entero-parasites worldwide; its prevalence is more common in developing countries. Giardia lamblia is considered one of the opportunistic parasites and its clinical manifestations represent the expression of host resistance and parasite virulence. As of chronic giardiasis cases were expected to worsen in immunosuppressed patients with development of variou...