نتایج جستجو برای: genomic dna of rye
تعداد نتایج: 21229200 فیلتر نتایج به سال:
In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...
Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...
the short arm of rye 1r chromosome (1rs) carries genes for resistance to diseases and pests. the presence of the 1rs arm also enhance adaptation, stress tolerance and yield potential of bread wheat. in the present study 17 bread wheat cultivars were screened to identify 1bl.1rs translocation (t1bl.1rs) by isoelectric focusing, c-banding and fluorescence in situ hybridization (fish) methods. iso...
specific local immunotherapy has been recently introduced as an alternative to classic subcutaneous immunotherapy in treatment of allergic rhinitis. in this study, the effects of sublingual immunotherapy (slit) on symptoms and medication score and skin prick test evaluation of patients with allergic rhinitis were investigated. in this placebo controlled trial, twenty four patients aged 5-18 yea...
Human blood DAT promoter methylation NucleoMag Blood 200 μL Transl Psychiatry 2015; 5:e648 Wiers et al. Effects of depressive symptoms and peripheral DAT methylation on neural reactivity to alcohol cues in alcoholism Automated isolation of genomic DNA from whole blood was carried out by the magnetic-bead-based NucleoMag Blood 200 μl Kit (Macherey Nagel, Düren, Germany). Venous blood PCR; genoty...
Background: Existence of bacterial host cell DNA contamination in biopharmaceutical products is a potential risk factor for patients receiving these drugs. Hence, the quantity of contamination must be controlled under the regulatory standards. Although different methods such as hybridization assays have been employed to determine DNA impurities, these methods are labor, intensive and rather exp...
Background: Impaired DNA repair mechanism is one of the main causes of tumor genesis. Study of intrinsic radiosensitivity of cancer patients in a non-target tissue (e.g. peripheral blood) might show the extent of DNA repair deficiency of cells in affected individuals and might be used a predictor of cancer predisposition. Methods: Initial radiation-induced DNA damage (ratio of Tail DNA/Head DN...
The recent years' problems of herbicide use have led a growing number of researchers to seek alternative methods that are less reliant on herbicides. This research was conducted to determine the effect of long-term crop rotation, mineral fertilizer and herbicide on the weed infestation and grain yield of winter rye during 2004 and 2005 at the long-term experimental site of the University of Tim...
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