Replication of human cytomegalovirus (HCMV) produces large DNA concatemers of head-to-tail-linked viral genomes that upon packaging into capsids are cut into unit-length genomes. The mechanisms underlying cleavage-packaging and the subsequent steps prior to nuclear egress of DNA-filled capsids are incompletely understood. The hitherto uncharacterized product of the essential HCMV UL52 gene was ...

The developmental expression of the antigen SO7, which has been previously shown to protect chickens against infection by several Eimeria species, was investigated. Using RT-PCR, mRNA for SO7 was found to be restricted primarily to unsporulated oocysts (0 hr). Western blot (WB) analysis with an antibody to recombinant SO7 (rbSO7) revealed expression of the protein from 6 to 72 hr (fully sporula...

background and objectives: baculovirus can be used as a vector in gene delivery system. viral envelope of baculovirus would display expressed protein/peptide and it could render as a potential vaccine delivery system. in this regard, the gene coding for a subunit of shiga toxin (stxa) from escherichia coli ( e. coli ) strain was cloned in a baculovirus expression system. stxa subunit has the ab...

objective: menstrual blood stromal stem cells (mbscs) share some phenotypic and functional similarities with mesenchymal stem cells (mscs). mscs are shown to inhibit either the function or generation of different immune cells, including dendritic cells (dcs). however, data regarding mbscs’ potential effects on immune system cells are elusive. here, we examine whether mbscs affect the generation...

Interaction of retrovirus vectors and endogenous retroviruses present in packaging cell lines and target cells may result in unwanted events, such as the formation of recombinant viruses and the mobilization of therapeutic vectors. Using sensitive reverse transcriptase PCR assays, we investigated human and murine gene therapy packaging cell lines for incorporation of endogenous retrovirus trans...

Background:The premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. Furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. So, it is necessary to tightly control IFN-γ pr...

this article reviews will focus on the concept and formation of micro particles (mps) in circulation and their role in transfusion medicine and immune system. mps are cell membrane derived vesicles which express markers of their parent cells and are found in circulation at low levels. exact functions of mps are unclear. in here, physiological almost all types of circulating mps including platel...

Sodium butyrate induces cell cycle arrest and apoptosis in bovine kidney epithelial cells primarily via downregulating cell cycle-related gene expression and enhancing expression of pro-apoptotic genes. The insulin-like growth factor (IGF) system plays an essential role in these processes. Understanding of regulation of insulin-like growth factor binding proteins (IGFBPs) by butyrate helps reve...