The therapeutic potential of hematopoietic stem cell (HSC) gene therapy can be fully exploited only by reaching efficient gene transfer into HSCs without compromising their biologic properties. Although HSCs can be transduced by HIV-derived lentiviral vectors (LVs) in short ex vivo culture, they display low permissivity to the vector, requiring cytokine stimulation to reach high-frequency trans...

Autologous leukemia cells engineered to express immune-stimulating molecules may be used to elicit antileukemia immune responses. Gene delivery to human B-precursor acute lymphoblastic leukemia (ALL) cells was investigated using the enhanced green fluorescent protein (EGFP) as a reporter gene, measured by flow cytometry. Transfection of the Nalm-6 and Reh B-precursor ALL leukemia cell lines wit...

Recent advances in our understanding of plant metabolism have highlighted the significance specialized metabolites regulation gene expression associated with biosynthetic networks. This opinion article focuses on molecular mechanisms small-molecule-mediated feedback at transcriptional level and its potential modes action, including metabolite signal perception, nature sensor, signaling transduc...

Graves’ disease (GD) is a multifactorial autoimmune with contribution from both genetic andepigenetic factors in its causation. Gene “protein tyrosine phosphatase non receptor 22” (PTPN22) animportant immune regulatory gene preventing hyper responsiveness of T cells by negatively regulatingtheir signal transduction. Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4) polymorphisms belong to themain deter...

The low levels of transduction of human hematopoietic stem cells (HSCs) with Moloney murine leukemia virus (MLV) vectors have been an obstacle to gene therapy for hematopoietic diseases. It has been demonstrated that lentivirus vectors are more efficient than MLV vectors at transducing nondividing cell lines as well as human CD34(+) cells and severe combined immunodeficiency disease repopulatin...

Abstract As an important member of the two‐component system (TCS), histidine kinases (HKs) play roles in various plant developmental processes and signal transduction response to a wide range biotic abiotic stresses. So far, HK gene family has not been investigated Gossypium . In this study, total 177 members were identified cotton. They further divided into seven groups, protein characteristic...

Abstract Background Gene therapy based on viral vectors is a promising new approach and offers great potential for the study treatment of cardiac diseases. Nucleic acid-based transfection human heart tissue could be considered valid translational alternative to use transgenic animal models in cardiovascular research. Purpose Here we explore Living Myocardial Slices (LMS) as platform nucleic the...

ABC transporters is a type of transmembrane widely found in prokaryotes and eukaryotes. They are involved plant signal transduction, secondary metabolite transport abiotic stress response. According to the transcriptome data Hydrangea, this study was proposed analyze members transporter gene family, physical chemical properties, structure, phylogeny expression patterns under aluminum using bioi...

Ex vivo gene editing of CD34+ hematopoietic stem and progenitor cells (HSPCs) offers great opportunities to develop new treatments for a number malignant non-malignant diseases. Efficient gene-editing in HSPCs has been achieved using electroporation and/or viral transduction deliver the CRISPR-complex, but cellular toxicity is drawback currently used methods. Nanoparticle (NP)-based strategies ...

Human hematopoietic stem cells (hHSCs) have enormous potential for clinical use in cell-based therapies, especially as a gene delivery system. Moreover, lentiviral transduction in stem cells is very often associated with low transduction efficiency and low levels of foreign gene expression. Therefore, it is important to analyze vector and promoter systems that can generate robust foreign gene e...