During nonpermissive infection by a T7 amber mutant in gene 1 (phage RNA polymerase-deficient), synthesis of the products of the phage genes 3 (endonuclease), 3, 5 (lysozyme), 5 (DNA polymerase), and 17 (serum blocking power) was shown to occur at about half the rate as during wild-type infection. This relatively high rate of expression of "late" genes (transcribed normally by the phage RNA pol...

A 23-yr-old man of Greek-Italian ancestry with mild anemia was found to be heterozygousforHbD(Punjab) fi12 glu -‘ gIn and fi-thalassemia. HbA was not detected upon electrophoresis of the subject’s hemolysate, and no synthesis of fiA globin was demonstrated after incubation of peripheral blood or bone marrow with 3H-Ieucine. The thalassemia gene was thus of the fi variety. The fiD/a synthesis ra...

RNA produced by the Simian Virus 40 (SV40) mutant tsA30 during lytic infection of kidney cells of African green monkeys was examined by RNA-DNA competition-hybridization. This mutant is temperature-sensitive in a function (gene A) that regulates synthesis of viral DNA. No detectable difference between mutant RNA synthesized at the permissive temperature (33 degrees ) and wild-type viral RNA was...

Background and Objective: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still an ongoing challenge in health system worldwide. Molecular detection methods including reverse transcription loop-mediated isothermal amplification (RT-LAMP) can help cutting off the transmission chain of the virus. In this study, prompt setting up of the RT-LAMP assays were investigated using ge...

Pseudouridine (Psi) synthases catalyze the isomerization of specific uridines in cellular RNAs to pseudouridines and may function as RNA chaperones. TruB is responsible for the Psi residue present in the T loops of virtually all tRNAs. The close homolog Cbf5/dyskerin is the catalytic subunit of box H/ACA snoRNPs. These carry out the pseudouridylation of eukaryotic rRNA and snRNAs. The 1.85 A re...

This paper presents a learning system devoted to learn rst order Horn clause concepts from ground concept examples and domain knowledge. Learning system employing rst order representation formalism encounters an enormous, possibly in nite, hypothesis search space. We propose a new topdown ILP system, ILPCS, which, in spite of looking for the target theory in the entire hypothesis space, control...

This paper presents the virtual gene genetic algorithm (vgGA) which is a generalization of traditional genetic algorithms that use binary linear chromosomes. In the vgGA, traditional one point crossover and mutation are implemented as arithmetic functions over the integers or reals that the chromosome represents. This implementation allows the generalization to virtual chromosomes of alphabets ...

background homocysteine is a sulfur-containing amino acid which formed (mainly in the liver) during the metabolism of methionine. prior studies indicated the important role of hyperhomocysteinemia in pathogenesis and progression of alcoholic liver disease, liver steatosis and cirrhosis. one of the most important mechanisms by which homocysteine promote the development of hepatic injury is oxida...