conclusions the results of this study demonstrated that the p450 cyp141 gene was successfully cloned into a pet26b plasmid vector as an expression vector. in this paper, for the first time in iran, this gene was cloned for more purposes, including the expression and purification of the recombinant cytochrome p450 cyp141 protein. results the cloning of p450 cyp141 gene was confirmed by the enzym...

abstract background: many disease susceptibility genes are large and consist of many exons in which point mutations are scattered throughout. scanning each exon individually represents a tedious task which can be time consuming and expensive. there has been increasing demand for rapid and accurate methods for full scanning of unknown point mutations in large multi-exon genes. gene assembling is...

the pituitary-specific transcription factor (pit1) gene is responsible for pituitary development and hormone secreting gene expression in mammals. pit-1 is studied as a candidate genetic marker for growth, carcass and also for milk yield traits. in dairy farm animals, the principal goal of the selection is the improvement of milk yield and composition. the genes of milk proteins and hormones ar...

In this study the genetic polymorphism of growth hormone (GH) gene as a candidate gene in livestock was investigated. Blood samples were randomly collected from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 365 bp region of exon 5 of the GH genewas amplified by polymerase chain reaction (PCR). PCR products were analyzed using single strand conformation polymorphism (...

Bovine Leukemia ProVirus: Evidence of Presence of Part of Gag Gene in Seminal Plasma of Naturally Infected Bulls It is of critical importance to understand the modalities of BLV presence in semen, especially with regard to artificial insemination (AI). Presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. In this study paired blood and semen sam...

b-lactoglobulin (Coded by the b-lg gene) is the major milk whey protein in ruminants. Studies have shown that this protein is polymorphic in many breeds of sheep as a result of a single base pair substitution in the b-lg gene that also gives rise to RsaI restriction fragment length polymorphism (RFLP). Blood samples were collected from 391 animals belonging to 5 Iranian and 6 Russian sheep bree...

background: methicillin resistant staphylococcus aureus (mrsa) is a common major human pathogen that causes hospital-acquired infections. characterization and typing of the staphylococcal cassette chromosome has led to a better understanding of mrsa infection cycle in hospital. the meca-associated hypervariable region size classifies mrsa isolates that colonized in nasal carriers. objectives: t...

aim : to evaluate the effect of active t. gondii tachyzoites and its products on the gene expression level of ifn-yr1 and ifn-yr2 in a murine model.   background : many studies have shown that the parasite toxoplasma gondii utilizes different mechanisms to inhibit the function of ifn-?, but the parasite effect on the function of ifn-yr1 and ifn-yr2 is still unclear. patients and methods : toxop...

Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. In this study, glutamate decarboxylase (gad) gene of a loca...

Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...