the mucus layer plays an important role as an intermediate for the protection of the gut against acidic chyme, digestive enzymes, and pathogens; in addition, it acts as a lubricant and facilitator of nutrient transportation. phytogenic compounds seem to promote intestinal mucus production. objectives: the current study was conducted to investigate the effects of low and high levels of energy an...

background: type ii diabetes is known as one of the most important, prevalent, and expensive diseases of mankind. late diagnosis and subsequent delayed initiation of treatment or surveillance of patients create a variety of problems for affected individuals. this has raised increasing concerns for public health authorities throughout the world. in the current study, we aimed to find a new appro...

b a c k g r o u n d  & aim: it is very helpful to classify and predict the clinical  category  of a sample based  on  its  gene  expression  profile.  this  study  was  conducted  to predict  tissues  of colorectal adenoma,  adenocarcinoma,  and  paired  normal  in  colon  based  on  microarray  data  using  nearest shrunken centroid method. methods   &  materials:    in  this  study,   the  co...

background calcium/calmodulin-dependent protein kinase iiα (camkiiα) may modulate the function of mu-opioid receptors by phosphorylation and therefore, be involved in development of morphine-induced analgesic tolerance. objectives the current study aimed to examine changes in gene expression of camkiiα in the lumbosacral cord and midbrain during induction of morphine analgesic tolerance. materi...

rna extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. although many rna extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. many studies have shown that trizol is an applicable material for the rna extracti...

background angiogenesis is an important biological process involved in the proliferation of endothelial cells, tumor growth and metastasis. vascular endothelial growth factor (vegf) is considered as a prominent regulator of angiogenesis which exerts the aforementioned effect(s) through its respective receptors (vegfr1 and vegfr2). vegf receptors are targeted as a therapeutic candidate for cance...

Background: Basal Cell Carcinoma (BCC) is a type of non-melanoma skin cancer. Alteration in gene expression is the important event that happens in cancer cell. Detection of this event is possible by proteomics techniques. Methods: Normal and tumor tissues were taken from BCC patient. Total proteins were purified by standard methods, and proteins were separated by two-dimensional electrophoresis...

background: various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. in the present study, we used a new protein expression system based on the iranian lizard leishmania, a trypanosomatid protozoan as a host, for the expression of lpg3 gene from leishmania infantum (l.infantum). methods: the lpg3 gene was cloned in the expression cass...