A partial genomic clone of the flavoprotein subunit of the mitochondrial enzyme, succinate dehydrogenase (EC 1.3.99.1) from Saccharomyces cerevisiae has been isolated. The partial clone was used to construct, by targeted gene disruption, a yeast mutant with a defective flavoprotein subunit gene. Submitochondrial membranes from the mutant are defective in activities requiring a functional succin...

We previously reported that disruption of the yjbI gene reduced virulence Staphylococcus aureus. In this study, we found in both silkworms and mice was restored by introducing yjbH but not to genes-disrupted mutants, suggesting yjbH, downstream a two-gene operon-yjbIH, is responsible for phenomenon. further observed decrease various surface-associated proteins changes cell envelope glycostructu...

Mycobacterium smegmatis inactivates rifampin by ribosylating this antibiotic. The gene responsible for this ability was cloned and was shown to confer low-level resistance to this antibiotic (MIC increase, about 12-fold) in related organisms. A 600-bp subclone responsible for ribosylating activity and resistance carried an open reading frame of 429 bp. Targeted disruption of the gene in M. smeg...

BACKGROUND: Among all common techniques in sitedirectedmutagenesis, λ Red recombinase system has beenwidely used to knock out chromosomal genes in bacteria. In thismethod, there is always the risk of DNA Linear digestion byhost's restriction enzymes that leads to the low frequency ofrecombination. OBJECTIVES:To overcome this, we constructeda recombinant vector to disrupt phoP gene in Salmonella...

Stricklett, Peter K., Raoul D. Nelson, and Donald E. Kohan. The Cre/loxP system and gene targeting in the kidney. Am. J. Physiol. 276 (Renal Physiol. 45): F651–F657, 1999.—The Cre/loxP and Flp/FRT systems mediate site-specific DNA recombination and are being increasingly utilized to study gene function in vivo. These systems allow targeted gene disruption in a single cell type in vivo, thereby ...

Self-disruptive Escherichia coli that produces foreign target protein was developed. E. coli was co-transformed with two vector plasmids, a target gene expression vector and a lysis gene expression vector. The lytic protein was produced after the expression of the target gene, resulting in simplification of the cell disruption process. In this study, the expression of cloned T4 phage gene e or ...

with the introduction of the use of seatbelts in cars, mortality following motor vehicle crashes has decreased significantly. however, two patterns of injuries, the ‘seatbelt sign’ and ‘seatbelt syndrome’ have emerged. injuries may consist of traumatic abdominal wall disruption. we present two cases of severe abdominal wall disruption caused by a seatbelt injury and treated with primary repair....

The Escherichia coli strain DH5α is used extensively in recombinant DNA technology. This is due in part to the endA gene mutation, which results in the reduction of endogenous levels of nuclease activity and increases the yield and quality of purified plasmid molecules. However, DH5α lacks the pyrF, leuB, and trpC mutations and is therefore not convenient for the selection of plasmids carrying ...

BACKGROUND The filamentous fungus Trichoderma reesei (Hypocrea jecorina) is an important source of cellulases for use in the textile and alternative fuel industries. To fully understand the regulation of cellulase production in T. reesei, the role of a gene known to be involved in carbon regulation in Aspergillus nidulans, but unstudied in T. reesei, was investigated. RESULTS The T. reesei or...

costochondral and rib grafts have long been used for reconstruction of lost condyle. donor site morbidity has less been under investigation and researched. pleural disruption pneumothorax and ugly scars are among the donor site complications. this study aims to determine the rate of pleural disruption following costochondral graft harvesting from the 4th to the 7th ribs of right and left ribs f...