نتایج جستجو برای: gel filtration
تعداد نتایج: 136090 فیلتر نتایج به سال:
A catalytically active species composed of a, y, and 8 subunits was isolated from phosphorylase kinase. Upon incubation of the holoenzyme (cusyS)4 with 1.8 M LiBr, 10 m~ LiBr, 10 m~ M&+ at 0 “C for 6-7 h and subsequent gel filtration and ion exchange chromatography, an active ayS complex was purified to apparent homogeneity by the criteria of gel filtration, disc gel electrophoresis under denat...
Analytical gel filtration A Superdex 75 column driven by an AKTA FPLC system (GE Healthcare) was used to separate monomeric and dimeric Venus-GRX2 immediately prior to spectral measurements and to evaluate the extent to which Venus-Gcn4 had been converted to monomer by enterokinase. All gel filtration experiments involving these proteins were performed using columns that had been equilibrated i...
We compared an immunochemical method specific for plasma proteins with a chemical method, in which interfering substances are separated by gel filtration and "total" urinary protein is determined by the biuret reaction followed by reaction of protein-bound copper with diethyldithiocarbamate after a second gel filtration to remove nonprotein-bound copper. More than 250 24-h urine samples were an...
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