Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid hydrolysate of casein, serves as the protein source. Beef extractives and starch are not added. Activated charcoal (Norit A or Norit SG) is included at 0.20% ...

The pleuropneumonia-like organisms (PPLO) are recognized as a group of rather fastidious microorganisms. Various peptones, infusions, and serum supplements have been examined by many investigators relating to the development of a medium satisfactory for the in vitro cultivation of these organisms (Morton et al., 1951; Edward, 1947; and others). However, few reports of examinations of the agar i...

Salmonella chromogenic medium (SCM; Oxoid, Basingstoke, United Kingdom), a new selective chromogenic medium, was compared to DCLS agar (Oxoid) for the detection and presumptive identification of Salmonella species from stool samples. This medium contains two chromogenic substrates, Magenta-cap (5-bromo-6-chloro-3-indolylcaprylate), which is hydrolyzed by Salmonella species to give magenta colon...