Subtilosin is a cyclical antimicrobial peptide produced by Bacillus amyloliquefaciens that has antimicrobial activity against the bacterial vaginosis-associated human pathogen Gardnerella vaginalis. The ability of subtilosin to inhibit G. vaginalis alone and in combination with the natural antimicrobial agents glycerol monolaurate (Lauricidin), lauric arginate, and ε-poly-L-lysine was tested us...

Twenty-five patients with uterine cervical cancer (Two cases stage O; four cases stage Ia; five cases stage Ib; one case stage IIa; ten cases stage IIb; two cases stage IIIa; one case stage IVa) served as the subjects. The bacterial flora inside the cervical cancers was investigated using the optimal technique. There was mixed abnormal aerobic and anaerobic bacterial flora in all subjects. The ...

INTRODUCTION Bacterial vaginosis is of clinical interest because of its possible causal relationship with complications during pregnancy, postpartum, and complications after surgery. METHODS Gram stain for clue cells and Gardnerella vaginalis culture methods were evaluated retrospectively in a microbiological medical laboratory for the first half of 2015. We were interested in the proportion ...

Gardnerella vaginalis, a pleomorphic Gram variable bacteria (1), with a Gram positive cell wall (2). that stains predominantly as Gram negative is associated in bacterial vaginosis (1). We have described a lysozyme-SDS-proteinase K method for the isolation of DNA from 250 ml Casman broth cultures requiring about 60 to 72 hours (3). However, this was not convenient for comparative restriction en...

Florence Bretelle ([email protected]) Florence Fenollar ([email protected]) Karine Baumstarck ([email protected]) Cécile Fortanier ([email protected]) Jean-François Cocallemen ([email protected]) Valérie Serazin ([email protected]) Didier Raoult ([email protected]) Pascal Auquier ([email protected]) Sandrine ...

In a study of Gardnerella vaginalis and anaerobic bacteria in non-specific vaginitis (NSV) and other genital disease 89 patients attending a genital medicine clinic had vaginal samples examined for conventional pathogens and for quantitative analysis of G vaginalis and aerobic and anaerobic bacterial flora. The overall incidence of G vaginalis was 20%; G vaginalis (mean concentration 7.0 log10/...