BACKGROUND AND OBJECTIVES Chromatin structure and thereby transcription is controlled by the level of acetylation of histones, which is determined by the balance between histone acetyl transferase (HAT) activity and histone deacetylase (HDAC) activity. HDAC inhibitors are a class of compounds able to regulate gene expression by modulating chromatin structure. There are two major classes of HDAC...

Background: The direct effect of some opioids on immune cells has been demonstrated. The aim of this study was to assess the apoptotic effect of opium on Jurkat T lymphocyte cells. Methods: Different concentrations of opium (2.86 × 10-3 to 2.86 × 10-11 g/ml) were added to 24-well plates containing 5 × 105 Jurkat cells. Apoptotic events were assessed after 6, 24, and 72 hours by flow-cytometric...

background: acute lymphoblastic leukemia (all) is the most common subtype of childhood cancer. chromosomal ab­normality, specially the replacement of chromosomal material is one of the main reasons in generating leukemia, wherein the kind of translocation play a key role in managing the remedy. the goal of the present study was to develop a reliable, rapid, and cost effective method to detect t...

Background: Microtubules (MT) are important components of cell cytoskeleton and play key roles in cell motility mitosis and meiosis. They are also the targets of several anticancer agents which indicating their importance in maintaining cell viability. Microtubular reorganization contributing to apoptotic morphology occurs in normal and neoplastic cells undergoing apoptosis induced by cytotoxic...

umbelliprenin is a prenylated compound, which belongs to the class of sesquiterpene coumarins. it is extracted from dried roots of ferula szwitsiana collected from the mountains of golestan forest (golestan province, north of iran). induction of apoptosis in jurkat t-cll cells has been previously shown. in this study, effect of umbelliprenin on proapoptotic caspases (caspase-8 and -9) and antia...

Flavonoids are naturally occurring antioxidants, with several flavonoids shown to have chemopreventive effects on cancer. We investigated the effects of the flavonoid acacetin on human T cell leukemia Jurkat cells. Acacetin inhibited the proliferation of Jurkat cells by inducing apoptosis in a concentration- and time-dependent manner. Acacetin-ind...

Acute leukemia is characterized by the accumulation of neoplastic cells in the bone marrow and peripheral blood. Currently, chemotherapy and differentiating agents have been used for the treatment of leukemia. Recently, plant extracts, either alone or in combination with chemo agents, have been proposed to be used for the treatment of cancers. The aim of the present research was to study the cy...

two igm monoclonal antibodies (mab) with strong reactivity for granulocytes and to a lesser extent for lurkat cell lines were established by immunizing balbi c mice with a histiocytic cell line (uy37). these two mabs (designated as 6c9 and 4c4) reacted with blast cells of t-acute lymphoblastic leukemia (t-all) and acute myelogenous leukemia (aml) patients as well as leukemic cells from patients...

Chromosome abnormalities that usually define high-risk acute lymphoblastic leukemia are the t(9;22)/ breakpoint cluster region protein-Abelson murine leukemia viral oncogene homolog 1, hypodiploid with < 44 chromosomes and 11q23/ myeloid/lymphoid leukemia gene rearrangements. The spectrum of acute lymphoblastic leukemia genetic abnormalities is nevertheless rapidly expanding. Therefore, newly d...

Precursor B-cell lymphoblastic leukemia is primarily a disease of children with most cases occurring before the age of six. Approximately 3000 new cases of lymphoblastic leukemia were reported in the United States in 2000. The presence of isochromosomes is a relatively unusual finding. In the few published studies available, isochromosomes occur in between 1-4% of call cases of lymphoblastic le...