Objective(s): In the current study, the effects of selected folk medicinal herbs were evaluated in D-galactose-induced aging in male mice.   Materials and Methods: Male BALB/c mice were randomly divided into 12 groups composing sham, control, and treated groups. Aging was induced by administration of D-galactose (500 mg/kg/day for 6 weeks). A positive control group was assigned...

Background and Objective: Aging is a natural phenomenon which can cause changes in most organs and cells. Numerous mechanisms including oxidative stress and free radical generation is involved in the progression of the aging process. Pomegranate seed oil (PSO), has different therapeutic properties including anti-oxidant and anti-inflammatory effects. In this research, the effect of PSO against ...

Past studies have suggested that mouse sperm surface galactosyltransferase may participate during fertilization by binding N-acetylglucosamine (GlcNAc) residues in the zona pellucida. In this paper, we examined further the role of sperm surface galactosyltransferase in mouse fertilization. Two reagents that specifically perturb sperm surface galactosyltransferase activity both inhibit sperm-zon...

  Background: Increased oxygen free radical and reduced glutathione level in the eye lens are important risk factor for cataract formation. The antioxidative property and increasing cellular and extra cellular glutathione level have been reported by several herbal medicines including silymarin.  Objective: In present interventional study Silybum marianum L. seed extract (silymarin) was tested a...

The present study aimed to evaluate the sensitivity and specificity of fucose some biochemical parameters, as an indicator therapeutic response Metformin in patients with recently diagnosed Polycystic Ovary Syndrome-PCOS undergoing treatment. This was conducted from 1/9/2022 1/11/2022. In 90 blood samples were collected women aged between (35-18) years old. Of these, 30 taken who had been polyc...

A kinetic microspectrophotometric assay for galactose1-phosphate uridylyitransferase (EC 2.7.7. 12) activity in erythrocytes is described. BlOOd is collected in ammonium heparinized microhematocrit tubes, centrifuged and the erythrocytes are lysed with water. Galactose-1-phosphate uridylyltransferase activity is determined by mixing 25 ul of hemolysate with a reagent consisting of galactose1-ph...

The reverse two-hybrid system has been developed to readily identify molecules or mutations that can disrupt protein-protein interactions in vivo. This system is generally based on the interaction-dependent activation of a reporter gene, whose product inhibits the growth of the engineered yeast cell. Thus, disruption of the interaction between the hybrid proteins can be positively selected beca...

In order to investigate the mechanism of inactivation of the galactose-fermenting system (“galactozymase”) after addition of glucose to galactose-grown cells of Saccharomyces cereuisiae the activities of the following enzymes of galactose utilization were assayed: the galactose uptake system, galactokinase, galact,ose-l-phosphate uridyltransferase, UDP-galactose-4-epimerase, and phosphoglucomut...

objective(s): silver nanoparticles (ag-nps) are one of the most widely used nanomaterials recently. despite the wide application of nanomaterials, there is limited information concerning their impact on human health and the environment. this study aimed to find the effects of ag-nps (40 nm) on blood serum, liver and kidney tissues of homing pigeons (columbia livia).materials and methods: columb...