The metabolic pathway by which beta-D-galactose is converted to glucose 1-phosphate is known as the Leloir pathway and consists of four enzymes. In most organisms, these enzymes appear to exist as soluble entities in the cytoplasm. In yeast such as Saccharomyces cerevisiae, however, the first and last enzymes of the pathway, galactose mutarotase and UDP-galactose 4-epimerase, are contained with...

In this study, a set of 400 ps molecular dynamics simulations were conducted to determine the stabilizing effect of O-glycosylation on the secondary structural integrity of the design α-loop-α motif. In general, glycosylation stabilizes the structural integrity of the model peptide because it decreases the opportunity for the surrounding water molecules to compete for the intramolecular hydroge...

Rhamnogalacturonan I (RGI) is a pectic polysaccharide composed of a backbone of alternating rhamnose and galacturonic acid residues with side chains containing galactose and/or arabinose residues. The structure of these side chains and the degree of substitution of rhamnose residues are extremely variable and depend on species, organs, cell types and developmental stages. Deciphering RGI functi...

Two immunologically reactive polysaccharides have been isolated from the cell walls and from culture filtrates of a filamentous alpha-hemolytic streptococcus provisionally designated capsular type 83. Both polysaccharides were purified by diethylaminoethyl-cellulose chromatography. Analysis indicates that the capsular polysaccharide consists of galactose and phosphorus, whereas the cell wall po...

Galactose-1-phosphate uridylyltransferase (GALT) is a key enzyme in galactose metabolism, particularly important in the neonatal period due to ingestion of galactose-containing milk. GALT deficiency results in the genetic disorder classic galactosemia, whose pathophysiology is still not fully elucidated. Whereas classic galactosemia has been hypothesized to result from GALT misfolding, a thorou...

Galactokinase (GalK) catalyses the first step of the Leloir pathway of galactose metabolism, the ATP-dependent phosphorylation of galactose to galactose-1-phosphate. In man, defects in galactose metabolism can result in disorders with severe clinical consequences, and deficiencies in galactokinase have been linked with the development of cataracts within the first few months of life. The crysta...

A capsular polysaccharide produced by Aerobacter aerogenes has been purified and shown to consist of galactose, mannose, and glucuronic acid in a molar ratio of 2: 1: 1, respectively. Partial acid hydrolysis liberated an aldobiouronic acid identified as 2-0-P-D-glucuronosyl-D-mannose. Analysis of capsular polysaccharide by Smith periodate degradation further indicated that all of the galactose ...

We demonstrate that the Na(+)-pump alpha-subunit polypeptide is glycosylated by using bovine milk galactosyltransferase, a specific enzyme which attaches galactose to terminal N-acetylglucosamine residues. The galactose acceptor sites are available for glycosylation only after permeabilization of right-side-out vesicles prepared from kidney outer medulla; therefore, the oligosaccharide moieties...