We evaluated the preventive effects of Terminalia chebula (T. chebula) aqueous extract on oxidative and antioxidative status in liver and kidney of aged rats compared to young albino rats. The concentrations of malondialdehyde (MDA), lipofuscin (LF), protein carbonyls (PCO), activities of xantione oxidase (XO), manganese-superoxide dismutase (MnSOD), catalase (CAT), glutathione peroxidase (GPx)...

This study investigated effect of repeated exposure to low doses of diuron in gilthead sea bream by measuring the activities of the following antioxidant enzymes: Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione transferase (GST), and DT-diaphorase (DTD). Examination of the lipid peroxidation (malondialdehyde contentMDA) and gluco...

This work evaluates the use of a plug of enzyme, migrating in an electrophoresis capillary under nondenaturing conditiong, to convert substrate (which may be injected onto the capillary as a separate plug or included in the electrophoresis buffer) to product. This concept is demonstrated using two systems: the irreversible oxidation of glucose-G-phosphate (glc-6-P) to 6-phosphogluconate using g...

Nutrition-induced effects on the activity of enzymes of lipogenesis, fatty acid synthase (FAS: EC 2.3.1.85), ATP citrate lyase (ACL: EC 4.1.3.8), malic enzyme (ME; EC 1.1.1.40), glucose-6-phosphate dehydrogenase (G6PDH: EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (PGDH; EC 1.1.1.44) were investigated in liver and interscapular brown adipose tissue (BAT) of rats. The lipogenic enzymes coul...

BACKGROUND This study evaluated the impact of Vernonia amygdalina (VA) on the transcription of key enzymes involved in cellular modulation of glucose in streptozotocin-induced diabetic rats in a bid to understand the possible anti-diabetic mechanism of VA. METHODS The chloroform fraction of VA (200 mg/kg and 400 mg/kg body weight) was administered to SDRs for 7 and 14 days. Thereafter, the ex...

Alzheimer's disease (AD) is the most prevalent form of dementia among the elderly. Although the underlying cause has yet to be established, numerous data have shown that oxidative stress is implicated in AD as well as in preclinical stages of AD, such as mild cognitive impairment (MCI). The oxidative stress observed in brains of subjects with AD and MCI may be due, either fully or in part, to i...

Steroids are a widely used class of drugs, and their hydroxylation modification has important pharmacological significance. Cytochrome P450 is the core enzyme for steroid molecules, but its use significantly restricted because low activity poor catalytic efficiency. In present study, we optimized codons human CYP17A1 (hCYP17A1) gene performed functional expression in Pichia pastoris GS115. The ...